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CCDC28A Recombinant monoclonal antibody, PBS Only (Detector) (86883-1-PBS)

Filter: AllWBIHCIF/ICCCytometric bead array sample testCytometric bead arrayAffinity

WB analysis using 86883-1-RR (same clone as 86883-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 86883-1-RR (CCDC28A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86883-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 86883-1-RR (same clone as 86883-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86883-1-RR (CCDC28A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86883-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 86883-1-RR (same clone as 86883-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CCDC28A antibody (86883-1-RR, Clone: 251922E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86883-1-PBS in a different storage buffer formulation.
Cytometric bead array sample test of MP02764-1
The mean CCDC28A concentration was determined to be 70.2 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.
Cytometric bead array standard curve of MP02764-1
Cytometric bead array standard curve of MP02764-1, CCDC28A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86883-2-PBS. Detection antibody: 86883-1-PBS. Standard: Ag6529. Range: 0.781-100 ng/mL.
Affinity and Kinetic Characterization of 86883-1-RR
Biolayer interferometry (BLl) kinetic assays of 86883-1-RR against Human CCDC28A were performed. The affinity constant is below 1 pM.

CCDC28A Recombinant monoclonal antibody, PBS Only (Detector)

Name: CCDC28A Recombinant monoclonal antibody, PBS Only (Detector)
Brand: Proteintech
SKU: 86883-1-PBS

CCDC28A Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 86883-1-PBS

Clone No.251922E2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

C6orf80, CC28A, CCRL1AP, Coiled-coil domain-containing protein 28A

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

86883-1-PBS targets CCDC28A as part of a matched antibody pair:

MP02764-1: 86883-2-PBS capture and 86883-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag6529 Product name: Recombinant human CCDC28A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-162 aa of BC000758 Sequence: MPKKNAIPVSKSTGFSNPASQSTSQRPKLKRVMKEKTKPQGGEGKGAQSTPIQHSFLTDVSDVQEMERGLLSLLNDFHSGKLQAFGNECSIEQMEHVRGMQEKLARLNLELYGELEELPEDKRKTASDSNLDRLLSDLEELNSSIQKLHLADAQDVPNTSAS Predict reactive species
Full Name	coiled-coil domain containing 28A
Calculated Molecular Weight	30 kDa
Observed Molecular Weight	25 kDa
GenBank Accession Number	BC000758
Gene Symbol	CCDC28A
Gene ID (NCBI)	25901
RRID	AB_3745184
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q8IWP9
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Coiled-coil domain-containing protein 28A (CCDC28A) is a translocation partner of nucleoporin 98 (NUP98) in acute leukemias, resulting in the creation of fusion genes and the expression of chimeric proteins. These fusion proteins, such as NUP98-CCDC28A, have been shown to promote myeloproliferative neoplasms (PMID: 22058212). Additionally, CCDC28A is essential for head-tail coupling stability, thereby ensuring sperm motility (PMID: 39500989).