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  • Western Blot (WB) analysis of various lysates using ATP6V1B2 Recombinant monoclonal antibody (87302-4-RR)

    WB analysis using 87302-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87302-4-RR (ATP6V1B2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ATP6V1B2 Recombinant monoclonal antibody (87302-4-RR)

    WB analysis of mouse brain using 87302-4-RR

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 87302-4-RR (ATP6V1B2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATP6V1B2 Recombinant monoclonal antibody (87302-4-RR)

    IF Staining of HeLa using 87302-4-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATP6V1B2 antibody (87302-4-RR, Clone: 252572H5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87302-4-RR

    Affinity of 87302-4-RR

    Biolayer interferometry (BLl) kinetic assays of 87302-4-RR against Human ATP6V1B2 were performed. The affinity constant is below 1 pM.

ATP6V1B2 Recombinant monoclonal antibody

ATP6V1B2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87302-4-RR
Clone No.252572H5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

ATP6B1B2, ATP6B2, HO57, VATB, V-ATPase subunit B 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, A549 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87302-4-RR targets ATP6V1B2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7174

Product name: Recombinant human ATP6V1B2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 162-511 aa of BC003100

Sequence: CRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFERNFIAQGPYENRTVFETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKH

Predict reactive species
Full Name ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 56-58 kDa
GenBank Accession NumberBC003100
Gene Symbol ATP6V1B2
Gene ID (NCBI) 526
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP21281
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification (PMID:18667600).

Protocols

Product Specific Protocols
IF protocol for ATP6V1B2 antibody 87302-4-RRDownload protocol
WB protocol for ATP6V1B2 antibody 87302-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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