Tested Applications
| Positive WB detected in | HUVEC cells, mouse brain tissue, U2OS cells, mouse heart tissue |
| Positive IF/ICC detected in | U2OS cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87333-1-RR targets TMEM201 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag21360 Product name: Recombinant human TMEM201 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 22-218 aa of BC137291 Sequence: VTACAAAGVLLYRIARRMKPTHTMVNCWFCNQDTLVPYGNRNCWDCPHCEQYNGFQENGDYNKPIPAQYLEHLNHVVSSAPSLRDPSQPQQWVSSQVLLCKRCNHHQTTKIKQLAAFAPREEGRYDEEVEVYRHHLEQMYKLCRPCQAAVEYYIKHQNRQLRALLLSHQFKRREADQTHAQNFSSAVKSPVQVILLR Predict reactive species |
| Full Name | transmembrane protein 201 |
| Calculated Molecular Weight | 666 aa, 72 kDa |
| Observed Molecular Weight | 43 kDa and 72 kDa |
| GenBank Accession Number | BC137291 |
| Gene Symbol | TMEM201 |
| Gene ID (NCBI) | 199953 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q5SNT2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TMEM201, also known as SAMP1 and NET5, is a member of the TMEM201 family. TMEM201 is a nuclear envelope transmembrane protein primarily localized to the inner nuclear membrane. It plays a crucial role in nuclear envelope architecture and is a component of the linker of nucleoskeleton and cytoskeleton (LINC) complex. TMEM201 is essential for proper nuclear division and nuclear membrane reorganization during mitosis (PMID: 35311970, PMID: 35311970). TMEM201 has two isoforms with the molecular weights of 43 kDa and 72 kDa, respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TMEM201 antibody 87333-1-RR | Download protocol |
| WB protocol for TMEM201 antibody 87333-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
