TMEM201 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 87333-1-RR (same clone as 87333-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87333-1-RR (TMEM201 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87333-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 87333-1-RR (same clone as 87333-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87333-1-RR (TMEM201 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87333-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 87333-1-RR (same clone as 87333-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TMEM201 antibody (87333-1-RR, Clone: 252547D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87333-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 87333-1-RR (same clone as 87333-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM201 antibody (87333-1-RR, Clone: 252547D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87333-1-PBS in a different storage buffer formulation.

TMEM201 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 87333-1-PBS

Clone No.252547D10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

NET5, SAMP1, Spindle-associated membrane protein 1

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Product Information

87333-1-PBS targets TMEM201 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag21360

  Product name: Recombinant human TMEM201 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 22-218 aa of BC137291

  Sequence: VTACAAAGVLLYRIARRMKPTHTMVNCWFCNQDTLVPYGNRNCWDCPHCEQYNGFQENGDYNKPIPAQYLEHLNHVVSSAPSLRDPSQPQQWVSSQVLLCKRCNHHQTTKIKQLAAFAPREEGRYDEEVEVYRHHLEQMYKLCRPCQAAVEYYIKHQNRQLRALLLSHQFKRREADQTHAQNFSSAVKSPVQVILLR

• Full Name: transmembrane protein 201
• Calculated Molecular Weight: 666 aa, 72 kDa
• Observed Molecular Weight: 43 kDa and 72 kDa
• GenBank Accession Number: BC137291
• Gene Symbol: TMEM201
• Gene ID (NCBI): 199953
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5SNT2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TMEM201, also known as SAMP1 and NET5, is a member of the TMEM201 family. TMEM201 is a nuclear envelope transmembrane protein primarily localized to the inner nuclear membrane. It plays a crucial role in nuclear envelope architecture and is a component of the linker of nucleoskeleton and cytoskeleton (LINC) complex. TMEM201 is essential for proper nuclear division and nuclear membrane reorganization during mitosis (PMID: 35311970, PMID: 35311970). TMEM201 has two isoforms with the molecular weights of 43 kDa and 72 kDa, respectively.