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PRPF19 Recombinant monoclonal antibody, PBS Only (Capture) (88331-1-PBS)

PRPF19 Recombinant monoclonal antibody, PBS Only (Capture)

Name: PRPF19 Recombinant monoclonal antibody, PBS Only (Capture), 100ug
Brand: Proteintech
SKU: 88331-1-PBS-100UG

PRPF19 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Cat No. 88331-1-PBS

Clone No.260837A11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Synonyms

EC:2.3.2.27, hPso4, Pre-mRNA-processing factor 19, PRP19, PSO4

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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Filter: AllWBSandwich ELISA sample testSandwich ELISAIF/ICC

WB analysis using 88331-1-RR (same clone as 88331-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 88331-1-RR (PRPF19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88331-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sandwich ELISA sample test of MP03457-2
The mean PRPF19 concentration was determined to be 545.95 ng/mL in HL-60 cell extract based on a 1.20 mg/mL extract load, 677.04 ng/mL in Raji cell extract based on a 1.50 mg/mL extract load, 371.27 ng/mL in Ramos cell extract based on a 1.20 mg/mL extract load, 278.50 ng/mL in HT-29 cell extract based on a 1.20 mg/mL extract load and 205.25 ng/mL in SKOV-3 cell extract based on a 1.20 mg/mL extract load.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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The mean PRPF19 concentration was determined to be 545.95 ng/mL in HL-60 cell extract based on a 1.20 mg/mL extract load, 677.04 ng/mL in Raji cell extract based on a 1.50 mg/mL extract load, 371.27 ng/mL in Ramos cell extract based on a 1.20 mg/mL extract load, 278.50 ng/mL in HT-29 cell extract based on a 1.20 mg/mL extract load and 205.25 ng/mL in SKOV-3 cell extract based on a 1.20 mg/mL extract load.
Sandwich ELISA standard curve of MP03457-2
Sandwich ELISA standard curve of MP03457-2, Human PRPF19 Recombinant Matched Antibody Pair - PBS only. 88331-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7700. 88331-4-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 88331-1-RR (same clone as 88331-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRPF19 antibody (88331-1-RR, Clone: 260837A11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 88331-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

88331-1-PBS targets PRPF19 as part of a matched antibody pair:

MP03457-2: 88331-1-PBS capture and 88331-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag7700 Product name: Recombinant human PRPF19 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 155-504 aa of BC008719 Sequence: GAGEPMDLGELVGMTPEIIQKLQDKATVLTTERKKRGKTVPEELVKPEELSKYRQVASHVGLHSASIPGILALDLCPSDTNKILTGGADKNVVVFDKSSEQILATLKGHTKKVTSVVFHPSQDLVFSASPDATIRIWSVPNASCVQVVRAHESAVTGLSLHATGDYLLSSSDDQYWAFSDIQTGRVLTKVTDETSGCSLTCAQFHPDGLIFGTGTMDSQIKIWDLKERTNVANFPGHSGPITSIAFSENGYYLATAADDSSVKLWDLRKLKNFKTLQLDNNFEVKSLIFDQSGTYLALGGTDVQIYICKQWTEILHFTEHSGLTTGVAFGHHAKFIASTGMDRSLKFYSL Predict reactive species
Full Name	PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)
Calculated Molecular Weight	55 kDa
Observed Molecular Weight	55 kDa
GenBank Accession Number	BC008719
Gene Symbol	PRPF19
Gene ID (NCBI)	27339
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9UMS4
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PRPF19 is involved in several processes, including DNA damage checkpoint signaling; cellular protein metabolic process; and mRNA splicing, via spliceosome.