FC experiment of human peripheral blood leukocytes using 98271-1-RR
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human IL-17RA/CD217 Rabbit Recombinant Antibody (98271-1-RR, Clone: 241891G7) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD16 (3G8). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human IL-17RA/CD217 Rabbit Recombinant Antibody (98271-1-RR, Clone: 241891G7) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD16 (3G8). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of human peripheral blood leukocytes using 98271-1-RR
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human IL-17RA/CD217 Rabbit Recombinant Antibody (98271-1-RR, Clone: 241891G7) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human IL-17RA/CD217 Rabbit Recombinant Antibody (98271-1-RR, Clone: 241891G7) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
Affinity and Kinetic Characterization of 98271-1-RR
Biolayer interferometry (BLl) kinetic assays of 98271-1-RR against Human IL-17RA/CD217 were performed. The affinity constant is 0.115 nM.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
98271-1-RR targets IL-17RA/CD217 in FC applications and shows reactivity with human samples.
FC experiment of human peripheral blood leukocytes using 98271-1-RR
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human IL-17RA/CD217 Rabbit Recombinant Antibody (98271-1-RR, Clone: 241891G7) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD16 (3G8). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of human peripheral blood leukocytes using 98271-1-RR
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human IL-17RA/CD217 Rabbit Recombinant Antibody (98271-1-RR, Clone: 241891G7) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
AFFINITY Figures
Affinity and Kinetic Characterization of 98271-1-RR
Biolayer interferometry (BLl) kinetic assays of 98271-1-RR against Human IL-17RA/CD217 were performed. The affinity constant is 0.115 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IL-17RA/CD217 Recombinant antibody
Catalog Number
98271-1-RR
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
