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Anti-Mouse TWEAKR/CD266 Rabbit Recombinant Antibody (98382-2-RR)

Anti-Mouse TWEAKR/CD266 Rabbit Recombinant Antibody

TWEAKR/CD266 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98382-2-RR

Clone No. 241646F11

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Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Synonyms

TNFRSF12, TWEAK R, CD266, FGF-inducible 14, Fgfrp2

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

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FC experiment of Transfected HEK-293T using 98382-2-RR
1x10^6 HEK-293T cells or TWEAKR/CD266 transfected HEK-293T cells were stained with 0.25 ug Anti-Mouse TWEAK R/TNFRSF12 Rabbit RecAb (98382-2-RR, Clone:241646F11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of Transfected HEK-293T using 98382-2-RR
1x10^6 HEK-293T cells(left) or TWEAK R/TNFRSF12 transfected HEK-293T cells(right) were stained with 0.25 ug Anti-Mouse TWEAK R/TNFRSF12 Rabbit RecAb (98382-2-RR, Clone:241646F11)(red) , or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 98382-2-RR
Biolayer interferometry (BLl) kinetic assays of 98382-2-RR against Mouse TWEAKR/CD266 were performed. The affinity constant is 0.983 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive FC detected in

Transfected HEK-293T cells

Recommended dilution

Application	Dilution
Flow Cytometry (FC)	FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98382-2-RR targets TWEAKR/CD266 in FC applications and shows reactivity with mouse samples.

Tested Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg1838 Product name: Recombinant Mouse TWEAKR/CD266 protein (rFc Tag) (HPLC-verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 28-80 aa of NM_013749.2 Sequence: EQAPGTSPCSSGSSWSADLDKCMDCASCPARPHSDFCLGCAAAPPAHFRLLWP Predict reactive species
Full Name	tumor necrosis factor receptor superfamily, member 12a
Calculated Molecular Weight	14kDa
GenBank Accession Number	NM_013749.2
Gene Symbol	Tnfrsf12a
Gene ID (NCBI)	27279
RRID	AB_3746027
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9CR75
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

TWEAKR (also known as CD266, FN14, TNFRSF12A) is a member of the TNF receptor superfamily which is activated by its ligand, the cytokine TWEAK (TNFSF12). TWEAKR is the smallest member of the TNFR superfamily. TweakR was first described as an FGF-inducible gene that played a role in fibroblast adhesion and migration. Subsequently, the induction of TweakR expression by other growth factors and/or upon tissue injury was observed in multiple cell types, including hepatocytes, endothelial cells, adipocytes, and cardiomyocytes. (PMID: 23073510, PMID: 24409185)