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MAN1A1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 78.1-5000 pg/mL

Cat no : MP02326-1

Synonyms

Alpha 1,2 mannosidase IA, Alpha-1,2-mannosidase IA, EC:3.2.1.113, Man(9)-alpha-mannosidase, Man9-mannosidase

Validation Data Gallery

  • Sandwich ELISA standard curve of MP02326-1, Human MAN1A1 Recombinant Matched Antibody Pair - PBS only. 86094-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag20060. 86094-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

    Sandwich ELISA standard curve of MP02326-1

    Sandwich ELISA standard curve of MP02326-1, Human MAN1A1 Recombinant Matched Antibody Pair - PBS only. 86094-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag20060. 86094-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Serum of six individual healthy human donors was measured. The MAN1A1 concentration of detected samples was determined to be 11.9 ng/mL with a range of 6.8-24.2 ng/mL.

    Sample test of MP02326-1

    Serum of six individual healthy human donors was measured. The MAN1A1 concentration of detected samples was determined to be 11.9 ng/mL with a range of 6.8-24.2 ng/mL.

  • Urine of six individual healthy human donors was measured. The MAN1A1 concentration of detected samples was determined to be 0.7 ng/mL with a range of ND-2.2 ng/mL.

    Sample test of MP02326-1

    Urine of six individual healthy human donors was measured. The MAN1A1 concentration of detected samples was determined to be 0.7 ng/mL with a range of ND-2.2 ng/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP02326-1 targets MAN1A1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: MAN1A1 Recombinant antibody, PBS Only (Capture) 86094-1-PBS (250444H2). 100 μg. Concentration 1 mg/ml.

Detection antibody: MAN1A1 Recombinant antibody, PBS Only (Detector) 86094-3-PBS (250444F10). 100 μg. Concentration 1 mg/ml.

Matched pair protein standard: Ag20060

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 86094-1-PBS
Tested Reactivity human
Immunogen MAN1A1 fusion protein Ag20060
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mannosidase, alpha, class 1A, member 1
Synonyms Alpha 1,2 mannosidase IA, Alpha-1,2-mannosidase IA, EC:3.2.1.113, Man(9)-alpha-mannosidase, Man9-mannosidase
GenBank accession numberBC065827
Gene symbol MAN1A1
UniprotP33908
Gene ID (NCBI) 4121
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 86094-3-PBS
Tested Reactivity human
Immunogen MAN1A1 fusion protein Ag20060
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mannosidase, alpha, class 1A, member 1
Synonyms Alpha 1,2 mannosidase IA, Alpha-1,2-mannosidase IA, EC:3.2.1.113, Man(9)-alpha-mannosidase, Man9-mannosidase
GenBank accession numberBC065827
Gene symbol MAN1A1
UniprotP33908
Gene ID (NCBI) 4121
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.