Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, MDCK cells, NIH/3T3 cells, PC-12 cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | mouse colon tissue, human breast cancer tissue, human prostate cancer tissue, human skeletal muscle tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse colon tissue |
| Positive IF/ICC detected in | HepG2 cells, A549 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:60000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26520-1-AP targets Vinculin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| Tested Reactivity | human, mouse, rat, canine |
| Cited Reactivity | human, mouse, rat, pig, rabbit, canine, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24942 Product name: Recombinant human Vinculin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 777-1066 aa of BC039174 Sequence: PKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ Predict reactive species |
| Full Name | vinculin |
| Calculated Molecular Weight | 1133 aa, 124 kDa |
| Observed Molecular Weight | 124 kDa |
| GenBank Accession Number | BC039174 |
| Gene Symbol | Vinculin |
| Gene ID (NCBI) | 7414 |
| RRID | AB_2868558 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P18206 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Vinculin antibody 26520-1-AP | Download protocol |
| IF protocol for Vinculin antibody 26520-1-AP | Download protocol |
| IHC protocol for Vinculin antibody 26520-1-AP | Download protocol |
| WB protocol for Vinculin antibody 26520-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Vinculin polyclonal antibody (Cat# 26520-1-AP) has 265 total citations published in journals including Nature Communications, Cell, Nature Cell Biology, Science Advances, Advanced Science, Blood, Cancer Discovery, and Molecular Cell. Associated research fields encompass oncology (hepatocellular, lung, breast, and prostate cancers), cell adhesion and mechanotransduction, ferroptosis, immunology, neuroscience, tissue engineering, inflammation, and metabolic regulation. Primary applications are Western blotting and immunofluorescence across human, mouse, and rat models.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Family with sequence similarity 114 member A1 orchestrates immune evasion in triple-negative breast cancer | ||
Signal Transduct Target Ther Fructose 1-phosphate inhibits mannose phosphate isomerase to suppress hepatocellular carcinogenesis | ||
Nat Nanotechnol DNA nanodevice for analysis of force-activated protein extension and interactions.
| ||
Cell Metab Electron transport chain inhibition increases cellular dependence on purine transport and salvage | ||
Cancer Discov Extrachromosomal DNA-Driven Oncogene Dosage Heterogeneity Promotes Rapid Adaptation to Therapy in MYCN-Amplified Cancers |
Reviews
What customers say
All 14 reviewers gave this antibody a perfect 5-star rating. It is most commonly used for Western Blot, with several users also reporting successful immunofluorescence staining. Reviewers consistently highlight clean, specific bands with high sensitivity—one user noted visible bands after only 0.5 seconds of exposure. The antibody performed well across diverse cell types including U2OS, HEK, HUVEC, fibroblasts, keratinocytes, and primary cells, as well as FFPE tissue sections. Recommended dilutions ranged from 1:100 (IF) to 1:40,000 (WB). Overall, users describe it as reliable, consistent, and excellent value for a loading control antibody.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Audrey (Verified Customer) (04-10-2026) | Works well for Western blot.
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Xiaoping (Verified Customer) (03-03-2026) | very good
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Ryan (Verified Customer) (02-04-2026) | Great antibody at good price.
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Nancy (Verified Customer) (01-30-2026) | Very good antibody. I always get consistent results when I use it for WB.
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Monica (Verified Customer) (11-14-2025) | Great antibody for Vinculin. I wanted a higher molecular weight loading control antibody. This worked so well. 1.5hr at RT for primary at secondary for 1 hr. Gave beautiful clean band. Very happy with this.
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JIN-FENG (Verified Customer) (10-08-2025) | The vinculin antibody works very well and very specific on my WB analysis.
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Tsimafei (Verified Customer) (09-22-2025) | Excellent and specific antibody
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Elham (Verified Customer) (06-18-2025) | Human airway smooth muscle cells fixed after 1 h of plating. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X 100. Blocked with 0.1% BSA and incubated with primary antibody overnight at 4C at 1:100 dilution. Following day cells were washed and incubated for 1 h with Alexa 594 at 1:400 dilution.
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Siavash (Verified Customer) (01-17-2025) | The antibody was used for immunofluorescence staining of 2D cultured cells fixed with 1%PFA and permeabilized with Triton X100. Cells were incubated overnight at +4 degrees celcius. The staining was visible in both epifluorescence microscope and confocal fluorescence microscope. Also, used for immunostaining of formalin-fixed paraffin-embedded (FFPE) skin of mouse. The antigen retrieval was citrate buffer. Primary antibody was incubated overnight at +4 degrees celcius.
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Sammy (Verified Customer) (12-04-2023) | Very good antibody for detection of vinculin by WB.
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Verdiana (Verified Customer) (11-03-2022) | Band at about 140kDa. Great antibody! Bands were visible just after 0.5sec of exposure! Clear bands.
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Bettina (Verified Customer) (08-03-2022) | Antibody worked well!
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Silvia (Verified Customer) (02-14-2022) | Good sharp clear band in western blot
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Bhuvanasundar (Verified Customer) (10-12-2021) | Immunofluorescence staining with this antibody worked very well.
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