Biotin-conjugated 5-methylcytosine Monoclonal antibody

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  IHC staining of mouse testis using Biotin-68301

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using Biotin-68301 (5-methylcytosine antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using Biotin-68301

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using Biotin-68301 (5-methylcytosine antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

5-methylcytosine Monoclonal Antibody for IHC

Cat No. Biotin-68301

Clone No.2C9G9

Host / Isotype

Mouse / IgG2b

Reactivity

5mc, chemical compound, m5c

Applications

IHC

Synonyms

m5C, 5mC

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Tested Applications

• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:1500-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-68301 targets 5-methylcytosine in IHC applications and shows reactivity with 5mc, chemical compound, m5c samples.

• Tested Reactivity: 5mc, chemical compound, m5c
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: 5-methylcytosine
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

In eukaryotes, 5-methylcytosine in DNA (5mC) and its oxidized derivatives (5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC)) are the most prominent modifications and have been suggested to contribute to epigenetic gene regulation through a variety of different mechanisms. m5C has also been found in mRNA, rRNA and tRNA of representative organisms from all kinds of species. As reversible epigenetic modifications, m5C modifications of RNA affect the fate of the modified RNA molecules and play important roles in various biological processes including RNA stability control, protein synthesis, and transcriptional regulation (PMID: 35365216). This antibody can recognize both 5mC in DNA and m5C in RNA.

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68301-1-IgDotBlot.pdf