m5C Monoclonal antibody
m5C Monoclonal Antibody for Dot Blot, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse, Rat, m5C
Applications
IHC, Dot Blot, ELISA
Conjugate
Unconjugated
CloneNo.
2C9G9
Cat no : 68301-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | mouse testis tissue, rat brain tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive ELISA detected in | compound |
Positive Dot Blot detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:10-1:100 |
DOT BLOT | DOT BLOT : 1:2500-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68301-1-Ig targets m5C in IHC, Dot Blot, ELISA applications and shows reactivity with Human, Mouse, Rat, m5C samples.
Tested Reactivity | Human, Mouse, Rat, m5C |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Compound |
Full Name | m5C |
Gene Symbol | |
Gene ID (NCBI) | |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
In eukaryotes, 5-methylcytosine in DNA (5mC) and its oxidized derivatives (5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC)) are the most prominent modifications and have been suggested to contribute to epigenetic gene regulation through a variety of different mechanisms. m5C has also been found in mRNA, rRNA and tRNA of representative organisms from all kinds of species. As reversible epigenetic modifications, m5C modifications of RNA affect the fate of the modified RNA molecules and play important roles in various biological processes including RNA stability control, protein synthesis, and transcriptional regulation (PMID: 35365216).
Protocols
Product Specific Protocols | |
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IHC protocol for m5C antibody 68301-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |