m5C Monoclonal antibody

m5C Monoclonal Antibody for IHC, ELISA, Dot Blot

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, m5C and More (2)

Applications

IF, IHC, ELISA, Dot Blot

Conjugate

Unconjugated

CloneNo.

2C9G9

Cat no : 68301-1-Ig

Synonyms



Tested Applications

Positive IHC detected inmouse testis tissue, rat brain tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive ELISA detected incompound
Positive Dot Blot detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
DOT BLOTDOT BLOT : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

68301-1-Ig targets m5C in IF, IHC, ELISA, Dot Blot applications and shows reactivity with Human, Mouse, Rat, m5C samples.

Tested Reactivity Human, Mouse, Rat, m5C
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Compound
Full Name m5C
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In eukaryotes, 5-methylcytosine in DNA (5mC) and its oxidized derivatives (5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC)) are the most prominent modifications and have been suggested to contribute to epigenetic gene regulation through a variety of different mechanisms. m5C has also been found in mRNA, rRNA and tRNA of representative organisms from all kinds of species. As reversible epigenetic modifications, m5C modifications of RNA affect the fate of the modified RNA molecules and play important roles in various biological processes including RNA stability control, protein synthesis, and transcriptional regulation (PMID: 35365216).

Protocols

Product Specific Protocols
IHC protocol for m5C antibody 68301-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Eur J Pharmacol

5- methylcytidine effectively improves spermatogenesis recovery in busulfan-induced oligoasthenospermia mice

Authors - Chengniu Wang
human

Cancer Biol Ther

Function of m5C RNA methyltransferase NOP2 in high-grade serous ovarian cancer

Authors - Shimin Yang