Tested Applications
| Positive WB detected in | mCherry-Flag/MYC/His Transfected 293T HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
HRP-84814 targets 6*His, His-Tag in WB, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | 6*His, His-Tag |
| Calculated Molecular Weight | 1 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_3749485 |
| Conjugate | HRP |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | HISTAG |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts to the 6*His tagged protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP 6*His, His-Tag antibody HRP-84814 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
HRP-84814 is the HRP-conjugated 6*His/His-Tag Recombinant Monoclonal Antibody (clone 241261A7). It has 2 indexed citations appearing in Nature Communications and Metabolic Engineering. The cited research spans complement system biology (membrane attack complex inhibition via C9-targeting miniproteins) and synthetic biology (post-transcriptional regulation using membraneless organelles).
| Species | Application | Title |
|---|---|---|
Nat Commun Design of miniprotein inhibitors targeting complement C9 to block membrane attack complex assembly. | ||
Metab Eng Engineering post-transcriptional regulation via synthetic membraneless organelles. |
Reviews
What customers say
HRP-84814 has one verified review from Anna K., who rated it 5/5 stars. She used it at a 1:2500 dilution for Western Blot (lot 21023098) to detect recombinant proteins containing a 6XHis tag on denaturing SDS-PAGE gels, with incubations performed in Every Blot Buffer. The reviewer reported excellent performance with no issues noted.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Anna (Verified Customer) (08-11-2026) | Denaturing SDS-PAGE of recombinant proteins containing 6XHis Tag, incubetionsin Every Blot Buffer
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