HRP-conjugated 6*his,His tag Recombinant monoclonal antibody

• 

  WB analysis of HEK-293T using HRP-84814

  mCherry-Flag/MYC/His Transfected 293T HEK-293T cells were subjected to SDS PAGE followed by western blot with HRP-84814 (6*his,His tag antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

6*his,His tag Uni-rAb^® Recombinant Antibody for WB

Cat No. HRP-84814

Clone No.241261A7

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB

Synonyms

6*His, His-Tag, 6*His, His Tag, His Tag, 241261A7, 6*His

---

Tested Applications

• Positive WB detected in: mCherry-Flag/MYC/His Transfected 293T HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

HRP-84814 targets 6*his,His tag in WB applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: 6*his,His tag
• Calculated Molecular Weight: 1 kDa
• Conjugate: HRP
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: HISTAG
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts to the 6*His tagged protein.