6*His, His-Tag Recombinant monoclonal antibody

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• 6*His, His-Tag Recombinant monoclonal antibody (84814-1-RR)

6*His, His-Tag Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 84814-1-RR

Clone No.241261A7

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

6*His, His Tag, His Tag, 241261A7, 6*His, 6*his,His tag

• 

  WB analysis using 84814-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84814-1-RR (6*his,His tag antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HEK-293 using 84814-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 6*his,His tag antibody (84814-1-RR, Clone: 241261A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of Transfected HEK-293T using 84814-1-RR

  1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.25 ug 6*his,His tag Recombinant antibody (84814-1-RR, Clone:241261A7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), and 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 84814-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84814-1-RR against a protein with N-terminal 6*His Tag were performed. The affinity constant is 1.69 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 84814-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84814-1-RR against a protein with C-terminal 6*His Tag were performed. The affinity constant is 26.1 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Transfected HEK-293T cells
• Positive IF/ICC detected in: HEK-293 cells
• Positive FC (Intra) detected in: Transfected HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84814-1-RR targets 6*His, His-Tag in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: 6*His, His-Tag
• Calculated Molecular Weight: 1 kDa
• RRID: AB_3672138
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: HISTAG
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts to the 6*His tagged protein.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		J Virol; GP2a I118 and GP4 D43 play critical roles in the attachment of PRRSV to the CD163 receptor: implications for anti-PRRSV infection targets; Authors - Guoqing Liu
		Cell Mol Life Sci; Rhapontigenin attenuates neurodegeneration in a parkinson's disease model by downregulating mtDNA-cGAS-STING-NF-κB-mediated neuroinflammation via PINK1/DRP1-dependent microglial mitophagy; Authors - Zhongqiang Su
	WB	Clin Transl Med; DNA-mediated UCP1 overexpression in adipose tissue: A promising anti-obesity gene therapy.; Authors - Ze-Wei Zhao
	WB,IF	Int J Biol Macromol; Genome-wide CRISPR screen identifies ALG5, ALG6, NF2, and FUT8 as key host proteins involved in transmissible gastroenteritis virus infection.; Authors - Zhhua Feng
	WB	PLoS Pathog; Two receptor-targeting mechanisms of lambda-like siphophage Gifsy-1 of Salmonella Typhimurium.; Authors - Xiaoli Zeng
	WB	Curr Mol Med; SSR2 Promotes Sorafenib Resistance Via Interacting with GPX4 to Inhibit Ferroptosis.; Authors - Zebing Song