Tested Applications
| Positive WB detected in | LNCaP cells, HEK-293 cells, HeLa cells, mouse brain tissue, rat brain tissue, ROS1728 cells, Caco-2 cells, HepG2 cells, Jurkat cells, NCI-H1299 cells, THP-1 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66888-1-Ig targets mTOR in WB, IHC, IF/ICC, IP, CoIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey, chicken, hamster, goat, zebra finches |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28395 Product name: Recombinant human MTOR protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 27-291 aa of NM_004958 Sequence: MSGLKSRNEETRAKAAKELQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQ Predict reactive species |
| Full Name | FK506 binding protein 12-rapamycin associated protein 1 |
| Calculated Molecular Weight | 289 kDa |
| Observed Molecular Weight | 250-289 kDa |
| GenBank Accession Number | NM_004958 |
| Gene Symbol | mTOR |
| Gene ID (NCBI) | 2475 |
| RRID | AB_2882219 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P42345 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for mTOR antibody 66888-1-Ig | Download protocol |
| IHC protocol for mTOR antibody 66888-1-Ig | Download protocol |
| IP protocol for mTOR antibody 66888-1-Ig | Download protocol |
| WB protocol for mTOR antibody 66888-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-mTOR antibody (Cat# 66888-1-Ig) has accumulated 824 citations across numerous high-impact journals, including Nature Communications, Nature Cell Biology, Molecular Cancer, Autophagy, Neuron, Cell Metabolism, Nature Cancer, Advanced Science, Cell Death & Disease, and Nature Metabolism. Associated research fields encompass autophagy, mTOR signaling, oncology, metabolism, neuroscience, inflammation, ferroptosis, immunology, cardiology, diabetes, hepatic disease, bone biology, stem cell biology, and biomaterials/tissue engineering, with Western blot being the predominant application.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther A two-strata energy flux system driven by a stress hormone prioritizes cardiac energetics | ||
Mol Cancer circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop. | ||
Mol Cancer A novel polypeptide encoded by the circular RNA ZKSCAN1 suppresses HCC via degradation of mTOR | ||
Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption | ||
Gastroenterology Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis | ||
Nat Cancer Low-protein diet enhances antitumor immunity in pancreatic cancer through microbiota-derived UDP-galactose |
Reviews
What customers say
All three reviews are 5-star ratings from Western Blot users. Reviewers consistently praise the antibody for producing strong, clean bands with excellent signal intensity. One user observed a clear band around 200 kDa in HEK293T cells at 1:1000 dilution. Another reported highly concentrated bands at 1:5000 dilution in cell lines. A third described it as a "lovely antibody" with very clean signal in PC3 cells. Overall, customers are satisfied with its specificity and performance across different cell types and dilutions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sun (Verified Customer) (01-05-2023) | strong band showed up around 200kDa region in HEK293T cells.
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Macarena (Verified Customer) (10-07-2022) | excellent band. Very concentrated.
|
Rachel (Verified Customer) (05-24-2021) | Lovely antibody, very clean good siganl Cell lysates were run on JESS Simple Western
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