Alpha 1B-Glycoprotein Recombinant monoclonal antibody, PBS Only (Detector)

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  WB analysis of human plasma using 86112-1-RR (same clone as 86112-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 86112-1-RR (A1BG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86112-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02261-1

  Serum of six individual healthy human donors was measured. The Alpha 1B-Glycoprotein concentration of detected samples was determined to be 44,277.2 ng/mL with a range of 31,684.6-51,508.3 ng/mL.

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  Sandwich ELISA standard curve of MP02261-1

  Sandwich ELISA standard curve of MP02261-1, Human Alpha 1B-Glycoprotein Recombinant Matched Antibody Pair - PBS only. 86112-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1511. 86112-1-PBS was HRP conjugated as the detection antibody. Range: 7.81-500 pg/mL.

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  IHC staining of human liver using 86112-1-RR (same clone as 86112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 86112-1-RR (Alpha 1B-Glycoprotein antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86112-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02261-1

  The mean A1BG/Alpha 1B-Glycoprotein concentration was determined to be 91.4 ug/mL in human serum.

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  Cytometric bead array standard curve of MP02261-1

  Cytometric bead array standard curve of MP02261-1, A1BG/Alpha 1B-Glycoprotein Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86112-2-PBS. Detection antibody: 86112-1-PBS. Standard: Eg1511. Range: 1.563-100 ng/mL.

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  Affinity and Kinetic Characterization of 86112-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86112-1-RR against Human Alpha 1B-Glycoprotein were performed. The affinity constant is 1.27 nM.

Alpha 1B-Glycoprotein Uni-rAb^® Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 86112-1-PBS

Clone No.250024H3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

A1BG, A1B, ABG, alpha 1 B glycoprotein, Alpha 1B glycoprotein

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Product Information

86112-1-PBS targets Alpha 1B-Glycoprotein as part of a matched antibody pair:

MP02261-1: 86112-2-PBS capture and 86112-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1511

  Product name: recombinant human A1BG protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 22-495 aa of NM_130786.4

  Sequence: AIFYETQPSLWAESESLLKPLANVTLTCQAHLETPDFQLFKNGVAQEPVHLDSPAIKHQFLLTGDTQGRYRCRSGLSTGWTQLSKLLELTGPKSLPAPWLSMAPVSWITPGLKTTAVCRGVLRGVTFLLRREGDHEFLEVPEAQEDVEATFPVHQPGNYSCSYRTDGEGALSEPSATVTIEELAAPPPPVLMHHGESSQVLHPGNKVTLTCVAPLSGVDFQLRRGEKELLVPRSSTSPDRIFFHLNAVALGDGGHYTCRYRLHDNQNGWSGDSAPVELILSDETLPAPEFSPEPESGRALRLRCLAPLEGARFALVREDRGGRRVHRFQSPAGTEALFELHNISVADSANYSCVYVDLKPPFGGSAPSERLELHVDGPPPRPQLRATWSGAVLAGRDAVLRCEGPIPDVTFELLREGETKAVKTVRTPGAAANLELIFVGPQHAGNYRCRYRSWVPHTFESELSDPVELLVAES

• Full Name: alpha-1-B glycoprotein
• Calculated Molecular Weight: 54kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: NM_130786.4
• Gene Symbol: A1BG
• Gene ID (NCBI): 1
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04217-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).