ABCF1 Polyclonal antibody, PBS Only

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• ABCF1 Polyclonal antibody, PBS Only (13950-1-PBS)

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  WB analysis using 13950-1-AP (same clone as 13950-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13950-1-AP (ABCF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 13950-1-AP (same clone as 13950-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 13950-1-AP (ABCF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 13950-1-AP (same clone as 13950-1-PBS)

  IP result of anti-ABCF1 (IP:13950-1-AP, 4ug; Detection:13950-1-AP 1:6000) with K-562 cells lysate 1360 ug. This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 13950-1-AP (same clone as 13950-1-PBS)

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 13950-1-AP (ABCF1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 13950-1-AP (same clone as 13950-1-PBS)

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 13950-1-AP (ABCF1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13950-1-AP (same clone as 13950-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ABCF1 antibody (13950-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13950-1-AP (same clone as 13950-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13950-1-AP (ABCF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13950-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ABCF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 13950-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

TNF-alpha-stimulated ABC protein, ATP-binding cassette sub-family F member 1, ATP-binding cassette 50, ATP binding cassette 50, ABCF 1

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Product Information

13950-1-PBS targets ABCF1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5061

  Product name: Recombinant human ABCF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 496-845 aa of BC034488

  Sequence: GWRKTLLIVSHDQGFLDDVCTDIIHLDAQRLHYYRGNYMTFKKMYQQKQKELLKQYEKQEKKLKELKAGGKSTKQAEKQTKEALTRKQQKCRRKNQDEESQEAPELLKRPKEYTVRFTFPDPPPLSPPVLGLHGVTFGYQGQKPLFKNLDFGIDMDSRICIVGPNGVGKSTLLLLLTGKLTPTHGEMRKNHRLKIGFFNQQYAEQLRMEETPTEYLQRGFNLPYQDARKCLGRFGLESHAHTIQICKLSGGQKARVVFAELACREPDVLILDEPTNNLDIESIDALGEAINEYKGAVIVVSHDARLITETNCQLWVVEEQSVSQIDGDFEDYKREVLEALGEVMVSRPRE

• Full Name: ATP-binding cassette, sub-family F (GCN20), member 1
• Calculated Molecular Weight: 92 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC034488
• Gene Symbol: ABCF1
• Gene ID (NCBI): 23
• RRID: AB_2242044
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NE71
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ABCF1, also known as ABC27, and ABC50, is a member of the superfamily of ATP-binding cassette (ABC) transporters and contains two ABC transporter domains. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). Unlike other members of the superfamily, ABCF1 lacks the transmembrane domains that are characteristic of most ABC transporters. ABCF1 may be regulated by tumor necrosis factor-alpha and play a role in the enhancement of protein synthesis and thus participate in the inflammatory processes(PMID: 9790762).