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ABHD14B Polyclonal antibody, PBS Only (20952-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis of L02 using 20952-1-AP (same clone as 20952-1-PBS)
L02 cells were subjected to SDS PAGE followed by western blot with 20952-1-AP (ABHD14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20952-1-PBS in a different storage buffer formulation.
WB analysis of mouse small intestine using 20952-1-AP (same clone as 20952-1-PBS)
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20952-1-AP (ABHD14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20952-1-PBS in a different storage buffer formulation.
IHC staining of human prostate hyperplasia using 20952-1-AP (same clone as 20952-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20952-1-AP (ABHD14B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20952-1-PBS in a different storage buffer formulation.
IHC staining of human prostate hyperplasia using 20952-1-AP (same clone as 20952-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20952-1-AP (ABHD14B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20952-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 20952-1-AP (same clone as 20952-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 20952-1-AP (ABHD14B antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20952-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 20952-1-AP (same clone as 20952-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 20952-1-AP (ABHD14B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20952-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 20952-1-AP (same clone as 20952-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ABHD14B antibody (20952-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 20952-1-PBS in a different storage buffer formulation.

ABHD14B Polyclonal antibody, PBS Only

Name: ABHD14B Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 20952-1-PBS

ABHD14B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 20952-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Abhydrolase domain-containing protein 14B, Alpha/beta hydrolase domain-containing protein 14B, CCG1-interacting factor B, CIB, EC:2.3.1.-

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

20952-1-PBS targets ABHD14B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15097 Product name: Recombinant human ABHD14B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-210 aa of BC007234 Sequence: MAASVEQREGTIQVQGQALFFREALPGSGQARFSVLLLHGIRFSSETWQNLGTLHRLAQAGYRAVAIDLPGLGHSKEAAAPAPIGELAPGSFLAAVVDALELGPPVVISPSLSGMYSLPFLTAPGSQLPGFVPVAPICTDKINAANYASVKTPALIVYGDQDPMGQTSFEHLKQLPNHRVLIMKGAGHPCYLDKPEEWHTGLLDFLQGLQ Predict reactive species
Full Name	abhydrolase domain containing 14B
Calculated Molecular Weight	210 aa, 22 kDa
Observed Molecular Weight	22-25 kDa
GenBank Accession Number	BC007234
Gene Symbol	ABHD14B
Gene ID (NCBI)	84836
RRID	AB_10733758
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96IU4
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

ABHD14B possessed the canonical ABHD fold, an invariant catalytic triad (Ser-His-Asp), and based on its protein sequence, was subsequently categorized as an outlying member of the metabolic serine hydrolase family. ABHD14B was able to transfer an acetyl group from post translationally modified protein lysine residue to coenzyme-A (CoA), thus making the biologically important acetyl-CoA.