ACAA2 Recombinant monoclonal antibody

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  WB analysis using 86161-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86161-3-RR (ACAA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 86161-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACAA2 antibody (86161-3-RR, Clone: 250814D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86161-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86161-3-RR against Human ACAA2 were performed. The affinity constant is 0.598 nM.

ACAA2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86161-3-RR

Clone No.250814D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

3-ketoacyl-CoA thiolase, mitochondrial, Acetyl CoA acyltransferase, Acetyl-CoA acetyltransferase, Acetyl-CoA acyltransferase, Acyl-CoA hydrolase, mitochondrial

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, Ramos cells, Caco-2 cells, NIH/3T3 cells, HSC-T6 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86161-3-RR targets ACAA2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1609

  Product name: Recombinant human ACAA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 98-397 aa of BC001918

  Sequence: SIVNGCQEICVKEAEVVLCGGTESMSQAPYCVRNVRFGTKLGSDIKLEDSLWVSLTDQHVQLPMAMTAENLAVKHKISREECDKYALQSQQRWKAANDAGYFNDEMAPIEVKTKKGKQTMQVDEHARPQTTLEQLQKLPPVFKKDGTVTAGNASGVADGAGAVIIASEDAVKKHNFTPLARIVGYFVSGCDPSIMGIGPVPAISGALKKAGLSLKDMDLVEVNEAFAPQYLAVERSLDLDISKTNVNGGAIALGHPLGGSGSRITAHLVHELRRRGGKYAVGSACIGGGQGIAVIIQSTA

• Full Name: acetyl-Coenzyme A acyltransferase 2
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC001918
• Gene Symbol: ACAA2
• Gene ID (NCBI): 10449
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42765
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.