CoraLite® Plus 488-conjugated ACPP Monoclonal antibody

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  IF Staining of human prostate cancer using CL488-60176

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite® Plus 488 ACPP antibody (CL488-60176, Clone: 1G2C9 ) at dilution of 1:200.

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  IF Staining of HeLa using CL488-60176

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 ACPP antibody (CL488-60176, Clone: 1G2C9 ) at dilution of 1:200, CL594-phalloidin (red).

ACPP Monoclonal Antibody for IF/ICC, IF-P

Cat No. CL488-60176

Clone No.1G2C9

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

IF/ICC, IF-P

Synonyms

PAP, ACP 3, acid phosphatase, prostate, Acid phosphatase 3, 5'-nucleotidase

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Tested Applications

• Positive IF-P detected in: human prostate cancer tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60176 targets ACPP in IF/ICC, IF-P applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8578

  Product name: Recombinant human ACPP protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 27-373 aa of BC007460

  Sequence: DRSVLAKELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELAGPVIPQDWSTECM

• Full Name: acid phosphatase, prostate
• Calculated Molecular Weight: 418 aa, 48 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC007460
• Gene Symbol: ACPP
• Gene ID (NCBI): 55
• RRID: AB_2919233
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15309
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).