CoraLite®594-conjugated ACPP Monoclonal antibody

ACPP Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G2C9

Cat no : CL594-60176

Synonyms

acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase

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Validation Data Gallery

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  IF Staining of human prostate cancer using CL594-60176

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite®594 ACPP antibody (CL594-60176, Clone: 1G2C9 ) at dilution of 1:200.

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  IF Staining of human prostate cancer using CL594-60176

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite®594 ACPP antibody (CL594-60176, Clone: 1G2C9 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human prostate cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60176 targets ACPP in IF applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACPP fusion protein Ag8578
• Full Name: acid phosphatase, prostate
• Calculated Molecular Weight: 418 aa, 48 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC007460
• Gene Symbol: ACPP
• Gene ID (NCBI): 55
• RRID: AB_2919899
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).