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alpha Actin Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 23660-1-AP

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Con: 33 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

alpha Actin Antibody WB mouse skeletal muscle tissue 23660-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 23660-1-AP (alpha Actin antibody) at dilution of 1:150000 incubated at room temperature for 1.5 hours

alpha Actin Antibody WB rat skeletal muscle tissue 23660-1-AP

rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23660-1-AP( alpha Actin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours

alpha Actin Antibody IHC human colon tissue 23660-1-AP

Immunohistochemistry of paraffin-embedded human colon slide using 23660-1-AP( alpha Actin antibody at dilution of 1:50

alpha Actin Antibody IHC human colon tissue 23660-1-AP

Immunohistochemistry of paraffin-embedded human colon slide using 23660-1-AP( alpha Actin antibody at dilution of 1:50

alpha Actin Antibody IP mouse skeletal muscle tissue 23660-1-AP

IP Result of anti-alpha Actin (IP:23660-1-AP, 4ug; Detection:23660-1-AP 1:4000) with mouse skeletal muscle tissue lysate 2200ug.

alpha Actin Antibody IF HepG2 cells 23660-1-AP

Immunofluorescent analysis of HepG2 cells using 23660-1-AP(alpha Actin antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG

Species specificity:
human, mouse, rat
Positive WB detected in:
rat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IP detected in:
mouse skeletal muscle tissue
Positive IHC detected in:
human colon tissue, human heart tissue, human skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:1000-1:8000
IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen Affinity purified
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
alpha Actin fusion protein Ag19151
Full name:
actin, alpha 1, skeletal muscle
Calculated molecular weight:
42 kDa
Observed molecular weight:
40-42 kDa
GenBank accession number:
BC012597
Gene ID (NCBI):
58
Gene symbol
ACTA1
Synonyms
ACTA, ACTA1, ACTA1 skeletal Muscle Actin, actin, Actin, alpha skeletal muscle, Alpha actin 1, ASMA, CFTD

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