Alpha Actin Polyclonal antibody

Alpha Actin Polyclonal Antibody for FC, IF, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

88

Cat no : 23660-1-AP

Synonyms

ACTA, ACTA1, ACTA1 skeletal Muscle Actin, actin, Actin, alpha skeletal muscle, Alpha Actin, Alpha actin 1, ASMA, CFTD, α actin

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Validation Data Gallery

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  WB analysis using 23660-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23660-1-AP (alpha Actin antibody) at dilution of 1:150000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 23660-1-AP

  IP Result of anti-alpha Actin (IP:23660-1-AP, 4ug; Detection:23660-1-AP 1:4000) with mouse skeletal muscle tissue lysate 2200ug.

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  IHC staining of human colon using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IHC staining of human colon using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IHC staining of human heart using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IHC staining of human heart using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IHC staining of human skeletal muscle using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IHC staining of human skeletal muscle using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IF Staining of HepG2 using 23660-1-AP

  Immunofluorescent analysis of HepG2 cells using 23660-1-AP (alpha Actin antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of C2C12 using 23660-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actin (23660-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human colon tissue, human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: C2C12 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 73 publications below
• IHC: See 1 publications below
• IF: See 16 publications below
• FC: See 1 publications below

Product Information

23660-1-AP targets Alpha Actin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha Actin fusion protein Ag19151
• Full Name: actin, alpha 1, skeletal muscle
• Calculated molecular weight: 42 kDa
• Observed molecular weight: 40-42 kDa
• GenBank accession number: BC012597
• Gene symbol: ACTA1
• Gene ID (NCBI): 58
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Acta Pharm Sin B; A novel SIRT6 activator ameliorates neuroinflammation and ischemic brain injury via EZH2/FOXC1 axis.; Authors - Tailin He
mouse	WB	Transl Res; Different roles of BAG3 in cardiac physiological hypertrophy and pathological remodeling.; Authors - Pengyu Jia
human	WB	Redox Biol; Mitochondrial dysfunction by TFAM depletion disrupts self-renewal and lineage differentiation of human PSCs by affecting cell proliferation and YAP response.; Authors - Yan Qi
	WB	Cardiovasc Diabetol; Down-regulation of WWP2 aggravates Type 2 diabetes mellitus-induced vascular endothelial injury through modulating ubiquitination and degradation of DDX3X; Authors - Shilong You
human	WB	J Hazard Mater; Chlorine disinfection elevates the toxicity of polystyrene microplastics to human cells by inducing mitochondria-dependent apoptosis.; Authors - Jing Qin
mouse	WB,IF	Phytomedicine; Aloe-emodin ameliorated MI-induced cardiac remodeling in mice via inhibiting TGF-β/SMAD signaling via up-regulating SMAD7; Authors - Jie Yu

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