Alpha Actin Polyclonal antibody

Name: Alpha Actin Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 23660-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23660-1-AP

Synonyms

ACTA, ACTA1, ACTA1 skeletal Muscle Actin, actin, Actin, alpha skeletal muscle, Alpha actin 1, ASMA, CFTD, α actin

WB analysis using 23660-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 23660-1-AP (alpha Actin antibody) at dilution of 1:150000 incubated at room temperature for 1.5 hours.
WB analysis of rat skeletal muscle using 23660-1-AP
rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23660-1-AP (alpha Actin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 23660-1-AP
Immunohistochemical analysis of paraffin-embedded human colon slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.
IHC staining of human colon using 23660-1-AP
Immunohistochemical analysis of paraffin-embedded human colon slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.
IP experiment of mouse skeletal muscle using 23660-1-AP
IP Result of anti-alpha Actin (IP:23660-1-AP, 4ug; Detection:23660-1-AP 1:4000) with mouse skeletal muscle tissue lysate 2200ug.
IF Staining of HepG2 using 23660-1-AP
Immunofluorescent analysis of HepG2 cells using 23660-1-AP (alpha Actin antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

Cat No. 23660-1-AP

Freight/Packing: -

Country/Region

Size/Concentration

Quantity

Positive WB detected in	rat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IP detected in	mouse skeletal muscle tissue
Positive IHC detected in	human colon tissue, human heart tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)	IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

WB	See 16 publications below
IF	See 3 publications below

The immunogen of 23660-1-AP is Alpha Actin Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Alpha Actin fusion protein Ag19151
Full Name	actin, alpha 1, skeletal muscle
Calculated molecular weight	42 kDa
Observed molecular weight	40-42 kDa
GenBank accession number	BC012597
Gene symbol	ACTA1
Gene ID (NCBI)	58
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Product Specific Protocols
WB protocol for Alpha Actin antibody 23660-1-AP	Download protocol
IHC protocol for Alpha Actin antibody 23660-1-AP	Download protocol
IF protocol for Alpha Actin antibody 23660-1-AP	Download protocol
IP protocol for Alpha Actin antibody 23660-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
rat	IF	Mol Med Rep Function of BRD4 in the pathogenesis of high glucose‑induced cardiac hypertrophy. Authors - Qian Wang View Article
human	WB	Acta Biochim Biophys Sin (Shanghai) Epigallocatechin-3-gallate inhibits angiotensin II-induced cardiomyocyte hypertrophy via regulating Hippo signaling pathway in H9c2 rat cardiomyocytes. Authors - Yuan Ma View Article
human	WB	Mol Ther Nucleic Acids PMID: 30925453 Authors - Gan Yu View Article
mouse	WB	Cell Death Dis Long noncoding RNA Neat1 modulates myogenesis by recruiting Ezh2. Authors - Shanshan Wang View Article
mouse	WB	Cell Death Dis Ginsenoside Rg3 promotes regression from hepatic fibrosis through reducing inflammation-mediated autophagy signaling pathway. Authors - Xiangxiang Liu View Article
human	WB	Exp Lung Res The roles of dipeptidyl peptidase-4 and its inhibitors in the regulation of airway epithelial-mesenchymal transition. Authors - Jingyi Sun View Article