Alpha Actin Polyclonal antibody

Alpha Actin Polyclonal Antibody for IF, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 23660-1-AP

Synonyms

ACTA, ACTA1, ACTA1 skeletal Muscle Actin, actin, Actin, alpha skeletal muscle, Alpha Actin, Alpha actin 1, ASMA, CFTD, α actin



Tested Applications

Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman colon tissue, human heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23660-1-AP targets Alpha Actin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Actin fusion protein Ag19151
Full Name actin, alpha 1, skeletal muscle
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 39-42 kDaa
GenBank Accession NumberBC012597
Gene Symbol ACTA1
Gene ID (NCBI) 58
RRIDAB_2879307
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Alpha Actin antibody 23660-1-APDownload protocol
IHC protocol for Alpha Actin antibody 23660-1-APDownload protocol
IF protocol for Alpha Actin antibody 23660-1-APDownload protocol
IP protocol for Alpha Actin antibody 23660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Acta Pharm Sin B

A novel SIRT6 activator ameliorates neuroinflammation and ischemic brain injury via EZH2/FOXC1 axis.

Authors - Tailin He
mouseWB

Transl Res

Different roles of BAG3 in cardiac physiological hypertrophy and pathological remodeling.

Authors - Pengyu Jia
humanWB

Redox Biol

Mitochondrial dysfunction by TFAM depletion disrupts self-renewal and lineage differentiation of human PSCs by affecting cell proliferation and YAP response.

Authors - Yan Qi
WB

Cardiovasc Diabetol

Down-regulation of WWP2 aggravates Type 2 diabetes mellitus-induced vascular endothelial injury through modulating ubiquitination and degradation of DDX3X

Authors - Shilong You
humanWB

J Hazard Mater

Chlorine disinfection elevates the toxicity of polystyrene microplastics to human cells by inducing mitochondria-dependent apoptosis.

Authors - Jing Qin
mouseWB,IF

Phytomedicine

Aloe-emodin ameliorated MI-induced cardiac remodeling in mice via inhibiting TGF-β/SMAD signaling via up-regulating SMAD7

Authors - Jie Yu