Alpha Actin Polyclonal antibody
Alpha Actin Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC,ELISA
Conjugate
Unconjugated
Cat no : 23660-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | rat skeletal muscle tissue, mouse skeletal muscle tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human colon tissue, human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 32 publications below |
| IF | See 4 publications below |
| FC | See 1 publications below |
Product Information
The immunogen of 23660-1-AP is Alpha Actin Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Alpha Actin fusion protein Ag19151 |
| Full Name | actin, alpha 1, skeletal muscle |
| Calculated molecular weight | 42 kDa |
| Observed molecular weight | 40-42 kDa |
| GenBank accession number | BC012597 |
| Gene symbol | ACTA1 |
| Gene ID (NCBI) | 58 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
| IHC protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
| IF protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
| IP protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Med Rep Function of BRD4 in the pathogenesis of high glucose‑induced cardiac hypertrophy. | ||
Acta Biochim Biophys Sin (Shanghai) Epigallocatechin-3-gallate inhibits angiotensin II-induced cardiomyocyte hypertrophy via regulating Hippo signaling pathway in H9c2 rat cardiomyocytes. | ||
Cell Death Dis Ginsenoside Rg3 promotes regression from hepatic fibrosis through reducing inflammation-mediated autophagy signaling pathway. | ||
Stem Cells Int 5-Azacytidine-Induced Cardiomyocyte Differentiation of Very Small Embryonic-Like Stem Cells. |
