Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, human colon tissue, human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue, mouse skeletal muscle tissue |
| Positive IF/ICC detected in | AC16 cells, H9C2 cells |
| Positive FC (Intra) detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23660-1-AP targets Alpha Actin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19151 Product name: Recombinant human ACTA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-377 aa of BC012597 Sequence: MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF Predict reactive species |
| Full Name | actin, alpha 1, skeletal muscle |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 39-42 kDaa |
| GenBank Accession Number | BC012597 |
| Gene Symbol | ACTA1 |
| Gene ID (NCBI) | 58 |
| RRID | AB_2879307 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P68133 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
| IF protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
| IHC protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
| IP protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
| WB protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Alpha Actin Polyclonal antibody (catalog no. 23660-1-AP) has 228 citations. These appear across journals including Signal Transduction and Targeted Therapy, Molecular Cancer, Cell Metabolism, Autophagy, Nature Communications, Redox Biology, Cell Death & Disease, Cancer Letters, Journal of Experimental Medicine, Oncogene, and Cell Reports, among others. Research fields span oncology (various cancers), cardiovascular disease, fibrosis, metabolism, ferroptosis, autophagy, and neuroscience, with applications mainly in Western blot, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Identification of miR-515-3p and its targets, vimentin and MMP3, as a key regulatory mechanism in esophageal cancer metastasis: functional and clinical significance. | ||
Mol Cancer CMTM6 overexpression confers trastuzumab resistance in HER2-positive breast cancer | ||
Autophagy The Valsa mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple | ||
Autophagy CCDC50 mediates the clearance of protein aggregates to prevent cellular proteotoxicity | ||
Nat Commun Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma |
Reviews
What customers say
The single review for this product is positive, rated 4 out of 5. The reviewer used it for Western Blot and reported that it performs well, noting it works effectively even at higher dilutions such as 1:10000. Overall, the feedback highlights the antibody's strong performance and good dilution flexibility for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Arun (Verified Customer) (07-28-2025) | Works great even wat higher dilutions 1:10000
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