ACTC1-specific Monoclonal antibody

ACTC1-specific Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F2B9

Cat no : 66125-1-Ig

Synonyms

ACTC, ACTC1, ACTC1 cardiac muscle 1, ACTC1 specific, Actin cardiac muscle specific, Actin, alpha cardiac muscle 1, actin, alpha, cardiac muscle 1, Alpha cardiac actin, CMD1R, CMH11



Tested Applications

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman heart tissue, human heart and human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2500-1:10000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66125-1-Ig targets ACTC1-specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name actin, alpha, cardiac muscle 1
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberNM_005159
Gene symbol ACTC1
Gene ID (NCBI) 70
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.

Protocols

Product Specific Protocols
WB protocol for ACTC1-specific antibody 66125-1-IgDownload protocol
IHC protocol for ACTC1-specific antibody 66125-1-IgDownload protocol
IF protocol for ACTC1-specific antibody 66125-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

J Am Heart Assoc

Postinfarction Hearts Are Protected by Premature Senescent Cardiomyocytes Via GATA 4-Dependent CCN 1 Secretion.

Authors - Sumei Cui