ACVR1 Monoclonal antibody

ACVR1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Mouse, Rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1F11B10

Cat no : 67417-1-Ig

Synonyms

activin A receptor, type I, Activin receptor like kinase 2, Activin receptor type 1, Activin receptor type I, ACTR I, ACTRI, ACVR1, ACVR1A, ACVRLK2, ALK 2, ALK2, FOP, SKR1, TSR I, TSRI

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Validation Data Gallery

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  WB analysis using 67417-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67417-1-Ig (ACVR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 67417-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67417-1-Ig (ACVR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67417-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67417-1-Ig (ACVR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67417-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67417-1-Ig (ACVR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 67417-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67417-1-Ig (ACVR1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67417-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67417-1-Ig (ACVR1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67417-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67417-1-Ig (ACVR1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67417-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67417-1-Ig (ACVR1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 67417-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ACVR1 antibody (67417-1-Ig, Clone: 1F11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 67417-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ACVR1 antibody (67417-1-Ig, Clone: 1F11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: pig brain tissue, rat brain tissue, NCI-H1299 cells, mouse brain tissue, JAR cells
• Positive IHC detected in: mouse heart tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67417-1-Ig targets ACVR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

• Tested Reactivity: Human, Pig, Mouse, Rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACVR1 fusion protein Ag13508
• Full Name: activin A receptor, type I
• Calculated Molecular Weight: 509 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC033867
• Gene Symbol: ACVR1
• Gene ID (NCBI): 90
• RRID: AB_2882657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACVR1 (activin receptor type I), also known as ALK2 or ACTRI, is a receptor for activin. It forms a stable complex with type II receptor after ligand binding. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling, and type II receptors are required for binding ligands and for expression of type I receptors. ACVR1 is expressed in many tissues including skeletal muscle and chondrocytes. It functions as a receptor for bone morphogenetic protein (BMP) and induces Indian hedgehog in chondrocytes during skeletal development. Mutations in ACVR1 gene are associated with fibrodysplasia ossificans progressive (PMID: 16642017).

Publications

Species	Application	Title
human	WB	J Cell Mol Med; Circulating exosomes repair endothelial cell damage by delivering miR-193a-5p.; Authors - Chang Cao