ADC Monoclonal antibody

ADC Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A2B11

Cat no : 67884-1-Ig

Synonyms

ADC, Antizyme inhibitor 2, ARGDC, arginine decarboxylase, AZI2, AZIN2, KIAA1945, ODC p, ODC paralogue, ODC1L, ODCP

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Validation Data Gallery

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  WB analysis of HEK-293T using 67884-1-Ig

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67884-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67884-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67884-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67884-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 67884-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67884-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 67884-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ADC antibody (67884-1-Ig, Clone: 1A2B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, mouse brain tissue, mouse cerebellum tissue, rat cerebellum tissue, HEK-293 cells, pig brain tissue
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67884-1-Ig targets ADC in WB, IF, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

• Tested Reactivity: Human, mouse, pig, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ADC fusion protein Ag31049
• Full Name: arginine decarboxylase
• Calculated Molecular Weight: 460 aa, 50 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC028128
• Gene Symbol: ADC
• Gene ID (NCBI): 113451
• RRID: AB_2918641
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Antizyme inhibitor 2 (AZIN2) is a member of the antizyme inhibitor family, witch is able to bind to AZs and inhibit their action on both ODC activity and polyamine uptake. AZIN2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of azin2 has also been observed in brains of patients with Alzheimer's disease (PMID: 19832840) . AZIN2 has 5 described insoforms produced by alternative splicing. The molecular weight of AZIN2 is about 50 kDa.