ADH1C Polyclonal antibody, PBS Only

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• ADH1C Polyclonal antibody, PBS Only (18897-1-PBS)

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  WB analysis using 18897-1-AP (same clone as 18897-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18897-1-AP (ADH1C antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 18897-1-AP (same clone as 18897-1-PBS)

  IP result of anti-ADH1C (IP:18897-1-AP, 4ug; Detection:18897-1-AP 1:1000) with mouse liver tissue lysate 4000 ug. This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18897-1-AP (same clone as 18897-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 18897-1-AP (same clone as 18897-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 18897-1-AP (same clone as 18897-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 18897-1-AP (same clone as 18897-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 18897-1-AP (same clone as 18897-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 18897-1-AP (same clone as 18897-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 18897-1-AP (same clone as 18897-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using ADH1C antibody 18897-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 18897-1-AP (same clone as 18897-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ADH1C antibody (18897-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

ADH1C Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 18897-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EC:1.1.1.1, ADH3

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Product Information

18897-1-PBS targets ADH1C in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13528

  Product name: Recombinant human ADH1C protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-374 aa of BC062476

  Sequence: MSTAGKVIKCKAAVLWELKKPFSIEEVEVAPPKAHEVRIKMVAAGICRSDEHVVSGNLVTPLPVILGHEAAGIVESVGEGVTTVKPGDKVIPLFTPQCGKCRICKNPESNYCLKNDLGNPRGTLQDGTRRFTCSGKPIHHFVGVSTFSQYTVVDENAVAKIDAASPLEKVCLIGCGFSTGYGSAVKVAKVTPGSTCAVFGLGGVGLSVVMGCKAAGAARIIAVDINKDKFAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGQLDTMMASLLCCHEACGTSVIVGVPPDSQNLSINPMLLLTGRTWKGAIFGGFKSKESVPKLVADFMAKKFSLDALITNVLPFEKINEGFDLLRSGKSIRTVLT

• Full Name: alcohol dehydrogenase 1C (class I), gamma polypeptide
• Calculated Molecular Weight: 375 aa, 40 kDa
• Observed Molecular Weight: 37-45 kDa
• GenBank Accession Number: BC062476
• Gene Symbol: ADH1C
• Gene ID (NCBI): 126
• RRID: AB_10596950
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P00326
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ADH1C(Alcohol dehydrogenase 1C) is also named as ADH3 and belongs to the zinc-containing alcohol dehydrogenase family.It catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde.The ADH1C gene is active in intestine and kidney in fetal and early postnatal life, and persists in the stomach and liver in adult life(PMID:4748759).