ADO Polyclonal antibody, PBS Only

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• ADO Polyclonal antibody, PBS Only (16479-1-PBS)

• 

  WB analysis of human brain using 16479-1-AP (same clone as 16479-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 16479-1-AP (ADO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16479-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16479-1-AP (same clone as 16479-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16479-1-AP (ADO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16479-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 16479-1-AP (same clone as 16479-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16479-1-AP (ADO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16479-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 16479-1-AP (same clone as 16479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 16479-1-AP (ADO antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16479-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 16479-1-AP (same clone as 16479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 16479-1-AP (ADO antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16479-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ADO Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 16479-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

2 aminoethanethiol dioxygenase, 2-aminoethanethiol dioxygenase, C10orf22, Cysteamine dioxygenase, EC:1.13.11.19

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Product Information

16479-1-PBS targets ADO in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9598

  Product name: Recombinant human ADO protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-270 aa of BC018660

  Sequence: MPRDNMASLIQRIARQACLTFRGSWGGRGASDRDAASGAEAPMQPGFPENLSKLKSLLTQLRAEDLNIAPRKATLQPLPPNLPPVTYMHIYETDGFSLGVFLLKSGTSIPLHDHPGMHGMLKVLYGTVRISCMDKLDAGGGQRPRALPPEQQFEPPLQPREREAVRPGVLRSRAEYTEASGPCILTPHRDNLHQIDAVEGPAAFLDILAPPYDPDDGRDCHYYRVLEPVRPKEASSSACDLPREVWLLETPQADDFWCEGEPYPGPKVFP

• Full Name: 2-aminoethanethiol (cysteamine) dioxygenase
• Calculated Molecular Weight: 270 aa, 30 kDa
• Observed Molecular Weight: 28 kDa-30 kDa
• GenBank Accession Number: BC018660
• Gene Symbol: ADO
• Gene ID (NCBI): 84890
• RRID: AB_2222204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96SZ5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ADO, or 2-aminoethanethiol dioxygenase, cysteamine dioxygenase, is a thiol dioxygenase in humans that adds two oxygen atoms to cysteamine, forming hypotaurine (PMID: 39637922). ADO has been reported to exhibit two distinct biological functions with a nonheme iron center. It catalyzes oxidation of both cysteamine in sulfur metabolism and N-terminal cysteine-containing proteins or peptides, such as regulator of G protein signaling 5 (RGS5) (PMID: 34508780). ADO is ubiquitously expressed with the highest levels in brain, heart, and skeletal muscle. Western blot analysis detected two bands at 28~30 kDa as indicated in publications (PMID: 17581819).