AFP Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

AFP Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D17

Cat no : 81083-1-PBS

Synonyms

AFP, Alpha 1 fetoprotein, Alpha fetoglobulin, alpha fetoprotein, FETA, HPAFP

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Validation Data Gallery

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  WB analysis of HepG2 using 81083-1-RR (same clone as 81083-1-PBS)

  WB result of AFP antibody (81083-1-RR; 1:14000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AFP transfected HepG2 cells. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 81083-1-RR (same clone as 81083-1-PBS)

  human placenta were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 81083-1-RR (same clone as 81083-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HuH-7 using 81083-1-RR (same clone as 81083-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81083-1-RR (same clone as 81083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81083-1-RR (AFP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81083-1-RR (same clone as 81083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81083-1-RR (AFP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81083-1-PBS targets AFP in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: AFP fusion protein Ag6089
• Full Name: alpha-fetoprotein
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 68-72 kDa
• GenBank Accession Number: BC027881
• Gene Symbol: AFP
• Gene ID (NCBI): 174
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult (PubMed:24120489). AFP is considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker (PubMed: 18669658).