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AFP Recombinant antibody
AFP Recombinant Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2D17
Cat no : 81083-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, human placenta tissue, HuH-7 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
81083-1-RR targets AFP in WB, IHC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | AFP fusion protein Ag6089 |
Full Name | alpha-fetoprotein |
Calculated Molecular Weight | 69 kDa |
Observed Molecular Weight | 68-72 kDa |
GenBank Accession Number | BC027881 |
Gene Symbol | AFP |
Gene ID (NCBI) | 174 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult (PubMed:24120489). AFP is considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker (PubMed: 18669658).
Protocols
Product Specific Protocols | |
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WB protocol for AFP antibody 81083-1-RR | Download protocol |
IHC protocol for AFP antibody 81083-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |