AID Polyclonal antibody, PBS Only

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• AID Polyclonal antibody, PBS Only (15642-1-PBS)

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  WB analysis using 15642-1-AP (same clone as 15642-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15642-1-AP (AID antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15642-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 15642-1-AP (same clone as 15642-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using AID antibody (15642-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15642-1-PBS in a different storage buffer formulation.

AID Polyclonal Antibody for WB, IF-P, Indirect ELISA

Cat No. 15642-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, Indirect ELISA

Synonyms

AICDA, Activation-induced cytidine deaminase, ARP2, CDA2, Cytidine aminohydrolase

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Product Information

15642-1-PBS targets AID in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8136

  Product name: Recombinant human AID protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-198 aa of BC006296

  Sequence: MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLDFGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNLSLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWEGLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL

• Full Name: activation-induced cytidine deaminase
• Calculated Molecular Weight: 198 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC006296
• Gene Symbol: AIDA/AID
• Gene ID (NCBI): 57379
• RRID: AB_2224236
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9GZX7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

AID is essential to all three genetic alterations required for antigen-specific immunoglobulin: class switch recombination (CSR), somatic bypermutation (SHM), and gene conversion. Expression of AID is normally restricted to B cells in the GC, where SHM and CSR occur. It is likely these represent AID monomer and homodimer that western blot detected two bands in purified AID from nuclear extracts, one at 52 kDa and the other at 26 kDa. (PMID:16280388)