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AK2 Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66127-1-Ig |CloneNo.: 3H2C10

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Con: 270 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

AK2 Antibody WB HeLa cells 66127-1-Ig

HeLa cells were subjected to SDS PAGE followed by western blot with 66127-1-Ig(AK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours

AK2 Antibody IHC human liver tissue 66127-1-Ig

Immunohistochemistry of paraffin-embedded human liver slide using 66127-1-Ig( AK2 Antibody) at dilution of 1:50

AK2 Antibody IHC human liver tissue 66127-1-Ig

Immunohistochemistry of paraffin-embedded human liver slide using 66127-1-Ig( AK2 Antibody) at dilution of 1:50

AK2 Antibody IF HepG2 cells 66127-1-Ig

Immunofluorescent analysis of HepG2 cells using 66127-1-Ig(AK2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG

AK2 Antibody FC HepG2 cells 66127-1-Ig

1X10^6 HepG2 cells were stained with .2ug AK2 antibody (66127-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

Species specificity:
human
Positive WB detected in:
HeLa cells, HEK-293 cells
Positive IHC detected in:
human liver tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells, HeLa cells
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:1000-1:8000
IHC : 1:20-1:200
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2a
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
AK2 fusion protein Ag17911
Full name:
adenylate kinase 2
Calculated molecular weight:
26 kDa
Observed molecular weight:
29 kDa
GenBank accession number:
BC009405
Gene ID (NCBI):
204
Gene symbol
AK2
Synonyms
adenylate kinase 2, ADK2, AK 2, AK2, ATP AMP transphosphorylase 2

Background

AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.


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