AK2 Monoclonal antibody

AK2 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF, FC

Conjugate

Unconjugated

CloneNo.

3H2C10

Cat no : 66127-1-Ig

Synonyms

adenylate kinase 2, ADK2, AK 2, AK2, ATP AMP transphosphorylase 2

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Validation Data Gallery

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  WB analysis using 66127-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66127-1-Ig (AK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66127-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66127-1-Ig (AK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50.

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  IHC staining of human liver using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50.

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  IHC staining of human heart using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 66127-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66127-1-Ig (AK2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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  IF Staining of HeLa using 66127-1-Ig

  Immunofluorescent analysis of HeLa cells using 66127-1-Ig (AK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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  FC experiment of HepG2 using 66127-1-Ig

  1X10^6 HepG2 cells were stained with .2ug AK2 antibody (66127-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human liver tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, HeLa cells
• Positive FC detected in: HepG2 cells

Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66127-1-Ig is AK2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: AK2 fusion protein Ag17911
• Full Name: adenylate kinase 2
• Calculated molecular weight: 26 kDa
• Observed molecular weight: 29 kDa
• GenBank accession number: BC009405
• Gene symbol: AK2
• Gene ID (NCBI): 204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.