AK2 Monoclonal antibody

AK2 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3H2C10

Cat no : 66127-1-Ig

Synonyms

adenylate kinase 2, ADK2, AK 2, AK2, ATP AMP transphosphorylase 2



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman liver tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66127-1-Ig is AK2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen AK2 fusion protein Ag17911
Full Name adenylate kinase 2
Calculated molecular weight 26 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC009405
Gene symbol AK2
Gene ID (NCBI) 204
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for AK2 antibody 66127-1-IgDownload protocol
IHC protocol for AK2 antibody 66127-1-IgDownload protocol
IF protocol for AK2 antibody 66127-1-IgDownload protocol
FC protocol for AK2 antibody 66127-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols