CoraLite®594-conjugated AK2 Monoclonal antibody
AK2 Monoclonal Antibody for FC (Intra), IF
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
IF, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
3H2C10
Cat no : CL594-66127
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of CL594-66127 is AK2 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | AK2 fusion protein Ag17911 |
Full Name | adenylate kinase 2 |
Calculated molecular weight | 26 kDa |
Observed molecular weight | 29 kDa |
GenBank accession number | BC009405 |
Gene symbol | AK2 |
Gene ID (NCBI) | 204 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
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IF protocol for CL594 AK2 antibody CL594-66127 | Download protocol |
FC protocol for CL594 AK2 antibody CL594-66127 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |