CoraLite®594-conjugated AK2 Monoclonal antibody

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  IF Staining of HepG2 using CL594-66127

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66127 (AK2 antibody) at dilution of 1:100.

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  FC experiment of HepG2 using CL594-66127

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human AK2 (CL594-66127, Clone:3H2C10) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

AK2 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL594-66127

Clone No.3H2C10

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

ATP:AMP phosphotransferase, ADK2, Adenylate monophosphate kinase, Adenylate kinase 2, mitochondrial, N-terminally processed, adenylate kinase 2

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66127 targets AK2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: AK2 fusion protein Ag17911
• Full Name: adenylate kinase 2
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC009405
• Gene Symbol: AK2
• Gene ID (NCBI): 204
• RRID: AB_2883521
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P54819
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.