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  • KD/KO Validated

AKT2 Polyclonal antibody

AKT2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17609-1-AP

Synonyms

AKT2, PKB beta, PKBB, PKBBETA, PRKBB, Protein kinase Akt 2, Protein kinase B beta, RAC BETA, RAC PK beta



Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human breast cancer tissue, human liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

17609-1-AP targets AKT2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKT2 fusion protein Ag11770
Full Name v-akt murine thymoma viral oncogene homolog 2
Calculated molecular weight 481 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC063421
Gene symbol AKT2
Gene ID (NCBI) 208
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis and their activation has been observed in a wide variety of cancers. AKT2 is mainly involved in cancer cell survival, apoptosis inhibition, migration and invasion(PMID:21979951). Defects in AKT2 are a cause of susceptibility to breast cancer (BC). AKT2 promotes metastasis of tumor cells without affecting the latency of tumor development. And defects in AKT2 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) and hypoinsulinemic hypoglycemia with hemihypertrophy (HIHGHH). The full length protein has four glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for AKT2 antibody 17609-1-APDownload protocol
IHC protocol for AKT2 antibody 17609-1-APDownload protocol
IP protocol for AKT2 antibody 17609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Respir Res

AKT2 drives cancer progression and is negatively modulated by miR-124 in human lung adenocarcinoma.

Authors - Ting Liu
  • KD Validated
humanWB

PLoS One

MicroRNA-137 Contributes to Dampened Tumorigenesis in Human Gastric Cancer by Targeting AKT2.

Authors - Liping Wu
humanWB

Front Cell Dev Biol

Regulation of MicroRNA-497-Targeting AKT2 Influences Tumor Growth and Chemoresistance to Cisplatin in Lung Cancer.

Authors - Lin Wang
humanWB

Oncotarget

Estrogen regulates miRNA expression: implication of estrogen receptor and miR-124/AKT2 in tumor growth and angiogenesis.

Authors - Cheng-Fei Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tom (Verified Customer) (11-03-2020)

Antibody diluted at 1:500 in blocking solution (5% BSA in TBS) and incubated overnight at 4 degrees.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HEK293T
AKT2 Antibody Western Blot validation (1:500 dilution) in HEK293T (Cat no:17609-1-AP)