• Featured Product
  • KD/KO Validated

AKT2 Polyclonal antibody

AKT2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17609-1-AP

Synonyms

AKT2, PKB beta, PKBB, PKBBETA, PRKBB, Protein kinase Akt 2, Protein kinase B beta, RAC BETA, RAC PK beta



Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human breast cancer tissue, human liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

17609-1-AP targets AKT2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKT2 fusion protein Ag11770
Full Name v-akt murine thymoma viral oncogene homolog 2
Calculated molecular weight 481 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC063421
Gene symbol AKT2
Gene ID (NCBI) 208
RRIDAB_2305350
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis and their activation has been observed in a wide variety of cancers. AKT2 is mainly involved in cancer cell survival, apoptosis inhibition, migration and invasion(PMID:21979951). Defects in AKT2 are a cause of susceptibility to breast cancer (BC). AKT2 promotes metastasis of tumor cells without affecting the latency of tumor development. And defects in AKT2 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) and hypoinsulinemic hypoglycemia with hemihypertrophy (HIHGHH). The full length protein has four glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for AKT2 antibody 17609-1-APDownload protocol
IHC protocol for AKT2 antibody 17609-1-APDownload protocol
IP protocol for AKT2 antibody 17609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO J

Asparagine reinforces mTORC1 signaling to boost thermogenesis and glycolysis in adipose tissues.

Authors - Yingjiang Xu
humanWB,IHC

Respir Res

AKT2 drives cancer progression and is negatively modulated by miR-124 in human lung adenocarcinoma.

Authors - Ting Liu
  • KD Validated
humanWB

Oncotarget

Estrogen regulates miRNA expression: implication of estrogen receptor and miR-124/AKT2 in tumor growth and angiogenesis.

Authors - Cheng-Fei Jiang
ratWB

Food Funct

l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways

Authors - Ling Lin
humanWB

Front Cell Dev Biol

miR-29b-3p Increases Radiosensitivity in Stemness Cancer Cells via Modulating Oncogenes Axis

Authors - Dong Pan
humanWB

Front Cell Dev Biol

Regulation of MicroRNA-497-Targeting AKT2 Influences Tumor Growth and Chemoresistance to Cisplatin in Lung Cancer.

Authors - Lin Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tom (Verified Customer) (11-03-2020)

Antibody diluted at 1:500 in blocking solution (5% BSA in TBS) and incubated overnight at 4 degrees.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HEK293T
AKT2 Antibody Western Blot validation (1:500 dilution) in HEK293T (Cat no:17609-1-AP)