Tested Applications
| Positive WB detected in | mouse liver tissue, rabbit liver tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68237-1-Ig targets ALDH2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.
| Tested Reactivity | Human, Mouse, Rat, Rabbit, Pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7463 Product name: Recombinant human ALDH2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 166-517 aa of BC002967 Sequence: DFFSYTRHEPVGVCGQIIPWNFPLLMQAWKLGPALATGNVVVMKVAEQTPLTALYVANLIKEAGFPPGVVNIVPGFGPTAGAAIASHEDVDKVAFTGSTEIGRVIQVAAGSSNLKRVTLELGGKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDIYDEFVERSVARAKSRVVGNPFDSKTEQGPQVDETQFKKILGYINTGKQEGAKLLCGGGIAADRGYFIQPTVFGDVQDGMTIAKEEIFGPVMQILKFKTIEEVVGRANNSTYGLAAAVFTKDLDKANYLSQALQAGTVWVNCYDVFGAQSPFGGYKMSGSGRELGEYGLQAYTEVKTVTVKVPQKNS Predict reactive species |
| Full Name | aldehyde dehydrogenase 2 family (mitochondrial) |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC002967 |
| Gene Symbol | ALDH2 |
| Gene ID (NCBI) | 217 |
| RRID | AB_2935324 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05091 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ALDH2 antibody 68237-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ALDH2 Monoclonal antibody (2G10F4), catalog number 68237-1-Ig, has 5 total citations. These appear in J Ginseng Res, Mol Nutr Food Res, Arch Biochem Biophys, PLoS One, and Biochem Biophys Res Commun. The associated research fields include alcohol-induced liver injury, septic hepatic injury, diabetic liver damage, lipid metabolism modulation, and pancreatic cancer. All cited applications are WB and IHC, with reactivity in human and mouse.
| Species | Application | Title |
|---|---|---|
J Ginseng Res Li-Ginseng powder protects against alcohol-induced liver injury by promoting acetaldehyde clearance and cellular homeostasis. | ||
Mol Nutr Food Res Protective Effect and Mechanism of L-Theanine on Acute Alcoholic Liver Injury in Mice | ||
Arch Biochem Biophys Inhibiting apoptosis and GSDME-mediated pyroptosis attenuates hepatic injury in septic mice | ||
PLoS One Artemether ameliorates type 1 diabetic liver injury alongside the associated defects in mitochondrial ultrastructure and central carbon metabolism. | ||
Biochem Biophys Res Commun The role of quercetin in modulating lipid metabolism and enhancing chemotherapy via the STAT3-CPT1B pathway in pancreatic cancer |
