ALDH2 Recombinant monoclonal antibody

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  WB analysis using 86429-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86429-5-RR (ALDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of C2C12 using 86429-5-RR

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using ALDH2 antibody (86429-5-RR, Clone: 251287E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A549 using 86429-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ALDH2 antibody (86429-5-RR, Clone: 251287E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 86429-5-RR

  Biolayer interferometry (BLl) kinetic assays of 86429-5-RR against Human ALDH2 were performed. The affinity constant is below 1 pM.

ALDH2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86429-5-RR

Clone No.251287E9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

ALDH 2, ALDH class 2, ALDH E2, ALDHE2, ALDH-E2

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Tested Applications

• Positive WB detected in: A549 cells, SK-BR-3 cells, HepG2 cells, mouse liver tissue, rat liver tissue
• Positive IF/ICC detected in: C2C12 cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86429-5-RR targets ALDH2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7452

  Product name:

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: of

  Sequence:

• Full Name: aldehyde dehydrogenase 2 family (mitochondrial)
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC002967
• Gene Symbol: ALDH2
• Gene ID (NCBI): 217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05091
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.