CoraLite® Plus 647-conjugated ALDH2 Recombinant monoclonal antibody

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  IF Staining of C2C12 using CL647-86429-5

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CoraLite® Plus 647 ALDH2 antibody (CL647-86429-5, Clone: 251287E9 ) at dilution of 1:200, CL488-phalloidin (green).

ALDH2 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL647-86429-5

Clone No.251287E9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

ALDH 2, ALDH class 2, ALDH E2, ALDHE2, ALDH-E2

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Tested Applications

• Positive IF/ICC detected in: C2C12 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-86429-5 targets ALDH2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7452

  Product name: Recombinant human ALDH2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 166-517 aa of BC002967

  Sequence: DFFSYTRHEPVGVCGQIIPWNFPLLMQAWKLGPALATGNVVVMKVAEQTPLTALYVANLIKEAGFPPGVVNIVPGFGPTAGAAIASHEDVDKVAFTGSTEIGRVIQVAAGSSNLKRVTLELGGKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDIYDEFVERSVARAKSRVVGNPFDSKTEQGPQVDETQFKKILGYINTGKQEGAKLLCGGGIAADRGYFIQPTVFGDVQDGMTIAKEEIFGPVMQILKFKTIEEVVGRANNSTYGLAAAVFTKDLDKANYLSQALQAGTVWVNCYDVFGAQSPFGGYKMSGSGRELGEYGLQAYTEVKTVTVKVPQKNS

• Full Name: aldehyde dehydrogenase 2 family (mitochondrial)
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC002967
• Gene Symbol: ALDH2
• Gene ID (NCBI): 217
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05091
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.