ALDH3A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ALDH3A1 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 15578-1-AP

Synonyms

Aldehyde dehydrogenase 3, ALDH3, ALDH3A1, ALDHIII

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Validation Data Gallery

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  WB analysis of A549 using 15578-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15578-1-AP (ALDH3A1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15578-1-AP

  WB result of ALDH3A1 antibody (15578-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH3A1 transfected A549 cells.

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  WB analysis of A549 using 15578-1-AP

  WB result of ALDH3A1 antibody (15578-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and ALDH3A1 knockout A549 cells.

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  IP experiment of A549 using 15578-1-AP

  IP result of anti-ALDH3A1(IP:15578-1-AP, 4ug; Detection:15578-1-AP 1:3000) with A549 cells lysate 1480 ug.

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  IF Staining of HEK-293T using 15578-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293T cells using ALDH3A1 antibody (15578-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293 using 15578-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-Human ALDH3A1 (15578-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IP detected in: A549 cells
• Positive IF detected in: HEK-293T cells
• Positive FC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• FC: See 2 publications below

Product Information

15578-1-AP targets ALDH3A1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALDH3A1 fusion protein Ag7977
• Full Name: aldehyde dehydrogenase 3 family, memberA1
• Calculated molecular weight: 50 kDa
• Observed molecular weight: 48 kDa
• GenBank accession number: BC004370
• Gene symbol: ALDH3A1
• Gene ID (NCBI): 218
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDH3A1(Aldehyde dehydrogenase, dimeric NADP-preferring), also named as ALDH3, is a member of the ALDH superfamily, which consists of NAD(P)+-dependent enzymes that catalyse the oxidation of a wide variety of endogenously produced and exogenous aldehydes to their corresponding acids. It is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UVlight-induced damage.(PMID:12943535). It can exsit as a dimer(PMID:16919242).

Publications

Species	Application	Title
human	WB	Mol Cell; Kynurenine importation by SLC7A11 propagates anti-ferroptotic signaling.; Authors - Alessandra Fiore
rat	IF	Nat Commun; Fiber reinforced GelMA hydrogel to induce the regeneration of corneal stroma.; Authors - Bin Kong
human	WB	Cell Chem Biol; Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons.; Authors - Amy Flor
human	IF	Antioxid Redox Signal; Elucidation of the AGR2 interactome in esophageal adenocarcinoma cells identifies a redox sensitive chaperone hub for the quality control of MUC-5AC.; Authors - Jack C Worfolk
human	IF,WB	ACS Biomater Sci Eng; Regulation of the Keratocyte Phenotype and Cell Behavior Derived from Human Induced Pluripotent Stem Cells by Substrate Stiffness; Authors - Lan Zhang
rat	IHC	Biomedicines; Human Umbilical Mesenchymal Stem Cell Xenografts Repair UV-Induced Photokeratitis in a Rat Model.; Authors - Yu-Show Fu

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C