ALDH3A1 Polyclonal antibody

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  WB analysis of A549 using 15578-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15578-1-AP (ALDH3A1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15578-1-AP

  WB result of ALDH3A1 antibody (15578-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH3A1 transfected A549 cells.

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  WB analysis of A549 using 15578-1-AP

  WB result of ALDH3A1 antibody (15578-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and ALDH3A1 knockout A549 cells.

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  IP experiment of A549 using 15578-1-AP

  IP result of anti-ALDH3A1 (IP:15578-1-AP, 4ug; Detection:15578-1-AP 1:3000) with A549 cells lysate 1480 ug.

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  IHC staining of human skin cancer using 15578-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15578-1-AP (ALDH3A1 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 15578-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15578-1-AP (ALDH3A1 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293T using 15578-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293T cells using ALDH3A1 antibody (15578-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293 using 15578-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-Human ALDH3A1 (15578-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

ALDH3A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15578-1-AP

Publications(27)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

AL3A1, Aldehyde dehydrogenase, dimeric NADP-preferring, ALDH3, ALDHIII, EC:1.2.1.5

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293T cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 13 publications below
• FC: See 1 publications below

Product Information

15578-1-AP targets ALDH3A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALDH3A1 fusion protein Ag7977
• Full Name: aldehyde dehydrogenase 3 family, memberA1
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC004370
• Gene Symbol: ALDH3A1
• Gene ID (NCBI): 218
• RRID: AB_2273745
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P30838
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDH3A1(Aldehyde dehydrogenase, dimeric NADP-preferring), also named as ALDH3, is a member of the ALDH superfamily, which consists of NAD(P)+-dependent enzymes that catalyse the oxidation of a wide variety of endogenously produced and exogenous aldehydes to their corresponding acids. It is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UVlight-induced damage.(PMID:12943535). It can exsit as a dimer(PMID:16919242).

Publications

Species	Application	Title
human	IF	Nat Commun; Impact of keratocyte differentiation on corneal opacity resolution and visual function recovery in male rats; Authors - Andri K Riau
human	WB	Mol Cell; Kynurenine importation by SLC7A11 propagates anti-ferroptotic signaling.; Authors - Alessandra Fiore
rat	IF	Nat Commun; Fiber reinforced GelMA hydrogel to induce the regeneration of corneal stroma.; Authors - Bin Kong
human	IHC	Adv Healthc Mater; Sustained Release of TPCA-1 from Silk Fibroin Hydrogels Preserves Keratocyte Phenotype and Promotes Corneal Regeneration by Inhibiting Interleukin-1β Signaling.; Authors - Wei Zhang
human	IF	Biofabrication; Development of a novel bioartificial cornea using 3D bioprinting based on electrospun micro-nanofibrous decellularized extracellular matrix; Authors - Shi-Yao Zhang
human	WB	Cell Chem Biol; Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons.; Authors - Amy Flor