ALG1 Polyclonal antibody, PBS Only

Name: ALG1 Polyclonal antibody, PBS Only,
Brand: Proteintech

ALG1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 12872-1-PBS

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Asparagine-linked glycosylation protein 1 homolog, Beta 1,4 mannosyltransferase, Beta-1,4-mannosyltransferase, Chitobiosyldiphosphodolichol beta-mannosyltransferase, EC:2.4.1.142

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

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Proteintech Guarantee

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 12872-1-AP (same clone as 12872-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 12872-1-AP (ALG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 12872-1-AP (same clone as 12872-1-PBS)
IP result of anti-ALG1 (IP:12872-1-AP, 4ug; Detection:12872-1-AP 1:500) with HeLa cells lysate 3080 ug. This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human gliomas using 12872-1-AP (same clone as 12872-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human gliomas using 12872-1-AP (same clone as 12872-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 12872-1-AP (same clone as 12872-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 12872-1-AP (same clone as 12872-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 12872-1-AP (same clone as 12872-1-PBS)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12872-1-AP (ALG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications
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Product Information

12872-1-PBS targets ALG1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3540 Product name: Recombinant human ALG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 157-464 aa of BC031095 Sequence: HNYGYSIMGLVHGPNHPLVLLAKWYEKFFGRLSHLNLCVTNAMREDLADNWHIRAVTVYDKPASFFKETPLDLQHRLFMKLGSMHSPFRARSEPEDPVTERSAFTERDAGSGLVTRLRERPALLVSSTSWTEDEDFSILLAALEKFEQLTLDGHNLPSLVCVITGKGPMREYYSRLIHQKHFQHIQVCTPWLEAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCCLPVCAVNFKCLHELVKHEENGLVFEDSEELAAQLQMLFSNFPDPAGKLNQFRKNLRESQQLRWDESWVRTVLPLVMDT Predict reactive species
Full Name	asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae)
Calculated Molecular Weight	464 aa, 53 kDa
Observed Molecular Weight	47-53 kDa
GenBank Accession Number	BC031095
Gene Symbol	ALG1
Gene ID (NCBI)	56052
RRID	AB_2289541
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BT22
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

ALG1 (asparagine-linked glycosylation 1) is a β-1,4-mannosyltransferase that catalyzes the committed step of N-linked glycosylation. Operating on the cytoplasmic face of the endoplasmic reticulum, ALG1 transfers the first mannose from GDP-mannose onto dolichol-pyrophosphate-GlcNAc₂, yielding Man₁GlcNAc₂-PP-dolichol. ALG1 is a 464-amino-acid protein with an N-terminal transmembrane domain that is essential for catalytic activity; deleting this domain abolishes mannosyltransferase function.