ALG1 Polyclonal antibody, PBS Only

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• ALG1 Polyclonal antibody, PBS Only (12872-1-PBS)

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  WB analysis of HeLa using 12872-1-AP (same clone as 12872-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12872-1-AP (ALG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12872-1-AP (same clone as 12872-1-PBS)

  IP result of anti-ALG1 (IP:12872-1-AP, 4ug; Detection:12872-1-AP 1:500) with HeLa cells lysate 3080 ug. This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12872-1-AP (same clone as 12872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12872-1-AP (same clone as 12872-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12872-1-AP (same clone as 12872-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12872-1-AP (ALG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ALG1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 12872-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

12872-1-PBS targets ALG1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3540

  Product name: Recombinant human ALG1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 157-464 aa of BC031095

  Sequence: HNYGYSIMGLVHGPNHPLVLLAKWYEKFFGRLSHLNLCVTNAMREDLADNWHIRAVTVYDKPASFFKETPLDLQHRLFMKLGSMHSPFRARSEPEDPVTERSAFTERDAGSGLVTRLRERPALLVSSTSWTEDEDFSILLAALEKFEQLTLDGHNLPSLVCVITGKGPMREYYSRLIHQKHFQHIQVCTPWLEAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCCLPVCAVNFKCLHELVKHEENGLVFEDSEELAAQLQMLFSNFPDPAGKLNQFRKNLRESQQLRWDESWVRTVLPLVMDT

• Full Name: asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae)
• Calculated Molecular Weight: 464 aa, 53 kDa
• Observed Molecular Weight: 47-53 kDa
• GenBank Accession Number: BC031095
• Gene Symbol: ALG1
• Gene ID (NCBI): 56052
• RRID: AB_2289541
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BT22
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.