CoraLite® Plus 488-conjugated AMPK Beta 2 Monoclonal antibody

AMPK Beta 2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D5C1

Cat no : CL488-66579

Synonyms

AMPK subunit beta 2, PRKAB2

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Validation Data Gallery

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  IF Staining of HeLa using CL488-66579

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 AMPK Beta 2 antibody (CL488-66579, Clone: 2D5C1 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66579 targets AMPK Beta 2 in IF applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AMPK Beta 2 fusion protein Ag5806
• Full Name: protein kinase, AMP-activated, beta 2 non-catalytic subunit
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC053610
• Gene Symbol: PRKAB2
• Gene ID (NCBI): 5565
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMPK beta 2 is a regulatory subunit of the AMP-activated protein kinase (AMPK), which is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK beta 2 may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles.