AMT Monoclonal antibody, PBS Only

AMT Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E7D4

Cat no : 67532-1-PBS

Synonyms

aminomethyltransferase, AMT, GCE, GCST, GCVT, NKH

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Validation Data Gallery

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  WB analysis of pig liver using 67532-1-Ig (same clone as 67532-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 67532-1-Ig (same clone as 67532-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 67532-1-Ig (same clone as 67532-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67532-1-Ig (same clone as 67532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67532-1-Ig (AMT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67532-1-Ig (same clone as 67532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67532-1-Ig (AMT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67532-1-Ig (same clone as 67532-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AMT antibody (67532-1-Ig, Clone: 3E7D4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67532-1-PBS targets AMT in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AMT fusion protein Ag26644
• Full Name: aminomethyltransferase
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 37-44 kDa
• GenBank Accession Number: BC007546
• Gene Symbol: AMT
• Gene ID (NCBI): 275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.