ANAPC5 Monoclonal antibody

ANAPC5 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Conjugate

Unconjugated

CloneNo.

2A7G5

Cat no : 67348-1-Ig

Synonyms

ANAPC5, APC5, Cyclosome subunit 5

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Validation Data Gallery

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  WB analysis using 67348-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67348-1-Ig (ANAPC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67348-1-Ig (ANAPC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67348-1-Ig (ANAPC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 67348-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ANAPC5 antibody (67348-1-Ig, Clone: 2A7G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human breast cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67348-1-Ig targets ANAPC5 in WB, IHC, IF applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ANAPC5 fusion protein Ag7409
• Full Name: anaphase promoting complex subunit 5
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 85 kDa, 72 kDa
• GenBank Accession Number: BC001081
• Gene Symbol: ANAPC5
• Gene ID (NCBI): 51433
• RRID: AB_2882606
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.