ANAPC5 Monoclonal antibody

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• ANAPC5 Monoclonal antibody (67348-1-Ig)

ANAPC5 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 67348-1-Ig Copied!

Clone No. 2A7G5

Publications (2)

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Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

2A7G5, Anaphase-promoting complex subunit 5, APC5, Cyclosome subunit 5

• 

  WB analysis using 67348-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67348-1-Ig (ANAPC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human breast cancer using 67348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67348-1-Ig (ANAPC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 67348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67348-1-Ig (ANAPC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 67348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67348-1-Ig (ANAPC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 67348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67348-1-Ig (ANAPC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse lung using 67348-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67348-1-Ig (ANAPC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse pancreas using 67348-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67348-1-Ig (ANAPC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat kidney using 67348-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67348-1-Ig (ANAPC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat lung using 67348-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 67348-1-Ig (ANAPC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human breast cancer using 67348-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ANAPC5 antibody (67348-1-Ig, Clone: 2A7G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human breast cancer tissue, rat lung tissue, mouse lung tissue, mouse pancreas tissue, human pancreas cancer tissue, rat kidney tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human breast cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67348-1-Ig targets ANAPC5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7409

  Product name: Recombinant human ANAPC5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-343 aa of BC001081

  Sequence: MASVHESLYFNPMMTNGVVHANVFGIKDWVTPYKIAVLVLLNEMSRTGEGAVSLMERRRLNQLLLPLLQGPDITLSKLYKLIEESCPQLANSVQIRIKLMAEGELKDMEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSFSQVFKLYTALQQYFQNGEKKTVEDADMELTSRDEGERKMEKEELDVSVREEEVSCSGPLSQKQAEFFLSQQASLLKNDETKALTPASLQKELNNLLKFNPDFAEAHYLSYLNNLRVQDVFSSTHSLLHYFDRLILTGAESKSNGEEGYGRSLRYAALNLAALHCRFGHYQQAELALQEAIRIAQESNDHVCLQHC

• Full Name: anaphase promoting complex subunit 5
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 85 kDa, 72 kDa
• GenBank Accession Number: BC001081
• Gene Symbol: ANAPC5
• Gene ID (NCBI): 51433
• RRID: AB_2882606
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9UJX4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

ANAPC5 Monoclonal antibody (2A7G5), catalog number 67348-1-Ig, has 2 citations appearing in Cancer Research and Advanced Science. The associated research fields are cancer immunology (CD24 membrane trafficking mediating antitumor immune evasion) and cell cycle regulation (CCDC68-promoted mitotic checkpoint activation). Both studies utilized the antibody for Western blot in human samples.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Res; Radiation-Enhanced CD24 Membrane Trafficking via GPI Anchoring Mediates Antitumor Immune Evasion.; Authors - Lingyi Kong ; KD Validated
human	WB	Adv Sci (Weinh); CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC; Authors - Qi Li