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CoraLite® Plus 647-conjugated ANKRD1 Monoclonal antibody

ANKRD1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, pig

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1F11F1

Cat no : CL647-67775

Synonyms

ALRP, ANKRD1, bA320F15.2, C 193, C193, Cardiac ankyrin repeat protein, CARP, CVARP, HA1A2, MCARP

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 647-conjugated ANKRD1 Monoclonal an (CL647-67775)

    IF Staining of A431 using CL647-67775

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 647 ANKRD1 antibody (CL647-67775, Clone: 1F11F1 ) at dilution of 1:1000.

Tested Applications

Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67775 targets ANKRD1 in IF applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ANKRD1 fusion protein Ag29974
Full Name ankyrin repeat domain 1 (cardiac muscle)
Calculated Molecular Weight 319 aa, 36 kDa
GenBank Accession NumberBC018667
Gene Symbol ANKRD1
Gene ID (NCBI) 27063
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ankyrin repeat domain-containing protein 1(ANKRD1) belongs to the conserved muscle ankyrin repeat protein(MARP) family, whose expression is induced in response to physiologic stress, injury and hypertrophy. Thus its expression mainly in activated vascular endothelial cells. ANKRD1 may regulate SMC proliferation through the CDKN1A pathway. It functions as a nuclear transcription repressor that regulate cardiac gene's expression

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 ANKRD1 antibody CL647-67775Download protocol
Standard Protocols
Click here to view our Standard Protocols