CoraLite®594-conjugated ANO2 Monoclonal antibody

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  IF Staining of mouse eye using CL594-67638

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CoraLite®594 ANO2 antibody (CL594-67638, Clone: 2F12G1 ) at dilution of 1:200.

ANO2 Monoclonal Antibody for IF-P

Cat No. CL594-67638

Clone No.2F12G1

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

IF-P

Synonyms

ANO2, anoctamin 2, C12orf3, DKFZp434P102, TMEM16B, Transmembrane protein 16B

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Tested Applications

• Positive IF-P detected in: mouse eye tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67638 targets ANO2 in IF-P applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: anoctamin 2
• Calculated Molecular Weight: 114 kDa
• GenBank Accession Number: NM_020373
• Gene Symbol: ANO2
• Gene ID (NCBI): 57101
• RRID: AB_2920167
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NQ90
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ANO2, also named as C12orf3 and TMEM16B, belongs to the anoctamin family. It acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. It may mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.