ANP32A Monoclonal antibody

ANP32A Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D8C5

Cat no : 67687-1-Ig

Synonyms

ANP32A, C15orf1, I1PP2A, LANP, MAPM, Mapmodulin, PHAP1, PHAPI, PP32



Tested Applications

Positive WB detected inA549 cells, HSC-T6 cells, HuH-7 cells, HeLa cells, Raji cells, Jurkat cells, Sp2/0 cells
Positive IHC detected inmouse brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67687-1-Ig targets ANP32A in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ANP32A fusion protein Ag8546
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
Calculated molecular weight 249 aa, 29 kDa
Observed molecular weight29 kDa
GenBank accession numberBC007200
Gene symbol ANP32A
Gene ID (NCBI) 8125
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ANP32A, an acidic leucine-rich nuclear phosphoprotein, is a member of the inhibitor of the histone acetyltransferase (INHAT) complex and preferentially binds to unmodified histone H3 tails in vitro. ANP32A is expressed in normal tissues as well as in pancreatic, breast and prostate cancer and other malignant tumors. ANP32A plays an important role in cell proliferation, apoptosis, transcriptional regulation, signal transduction and other processes.

Protocols

Product Specific Protocols
WB protocol for ANP32A antibody 67687-1-IgDownload protocol
IHC protocol for ANP32A antibody 67687-1-IgDownload protocol
IF protocol for ANP32A antibody 67687-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols