CoraLite®594-conjugated ANP32A Monoclonal antibody

ANP32A Monoclonal Antibody for IF, WB

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D8C5

Cat no : CL594-67687

Synonyms

ANP32A, C15orf1, I1PP2A, LANP, MAPM, Mapmodulin, PHAP1, PHAPI, PP32

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Validation Data Gallery

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  WB analysis using CL594-67687

  Various lysates were subjected to SDS PAGE followed by western blot with CL594-67687 (ANP32A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using CL594-67687

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®594 ANP32A antibody (CL594-67687, Clone: 2D8C5 ) at dilution of 1:200, CL488-Phalloidin (green).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, Raji cells
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67687 targets ANP32A in WB, IF applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ANP32A fusion protein Ag8546
• Full Name: acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
• Calculated Molecular Weight: 249 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC007200
• Gene Symbol: ANP32A
• Gene ID (NCBI): 8125
• RRID: AB_2920172
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANP32A, an acidic leucine-rich nuclear phosphoprotein, is a member of the inhibitor of the histone acetyltransferase (INHAT) complex and preferentially binds to unmodified histone H3 tails in vitro. ANP32A is expressed in normal tissues as well as in pancreatic, breast and prostate cancer and other malignant tumors. ANP32A plays an important role in cell proliferation, apoptosis, transcriptional regulation, signal transduction and other processes.