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  • KD/KO Validated

Annexin A1 Polyclonal antibody

Annexin A1 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 21990-1-AP


Annexin 1, annexin A1, Annexin I, ANX1, ANXA1, Calpactin 2, Calpactin II, Chromobindin 9, Lipocortin I, LPC1

Tested Applications

Positive WB detected inA431 cells, multi-cells, MDA-MB-453s cells, HeLa cells, U-937 cells, HepG2 cells, MCF-7 cells, C6 cells, A549 cells, K-562 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman oesophagus tissue, human kidney tissue, human small intestine tissue, human ovary tumor tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells, A431 cells
Positive FC detected inNIH/3T3 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21990-1-AP targets Annexin A1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin A1 fusion protein Ag17273
Full Name annexin A1
Calculated Molecular Weight 346 aa, 39 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC001275
Gene Symbol ANXA1
Gene ID (NCBI) 301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.


Product Specific Protocols
WB protocol for Annexin A1 antibody 21990-1-APDownload protocol
IHC protocol for Annexin A1 antibody 21990-1-APDownload protocol
IF protocol for Annexin A1 antibody 21990-1-APDownload protocol
IP protocol for Annexin A1 antibody 21990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Guorong (Verified Customer) (05-23-2022)

excellent with 293T cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: 293T
Annexin A1 Antibody Western Blot validation (1:2000 dilution) in 293T (Cat no:21990-1-AP)