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Annexin A1 Polyclonal antibody (21990-1-AP)

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Annexin A1 Polyclonal antibody

Annexin A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 21990-1-AP

Publications (72)

Reviews (2)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

ANXA1, Annexin 1, Annexin Ac2-26, Annexin I, Annexin-1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 21990-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 21990-1-AP
WB result of Annexin A1 antibody (21990-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A1 transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 21990-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 21990-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of multi-cells using 21990-1-AP
WB result of 21990-1-AP (Annexin A1 antibody).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MDA-MB-453s using 21990-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of U-937 using 21990-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of A431 using 21990-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 21990-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 21990-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of C6 using 21990-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 21990-1-AP
IP result of anti-Annexin A1 (IP:21990-1-AP, 4ug; Detection:21990-1-AP 1:20000) with HeLa cells lysate 1120 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human oesophagus using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21990-1-AP (Annexin A1 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21990-1-AP (Annexin A1 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human oesophagus using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21990-1-AP (Annexin A1 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary tumor using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary tumor using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human small intestine using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human small intestine using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human oesophagus using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human oesophagus using 21990-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of A549 using 21990-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Annexin A1 antibody (21990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of A431 using 21990-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Annexin A1 antibody (21990-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of NIH/3T3 using 21990-1-AP
1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Human Annexin A1 (21990-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of Jurkat using 21990-1-AP
1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Annexin A1 Polyclonal antibody (21990-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for Annexin A1 in ?

Overview

Tested Applications
Product Information
Publications (72)
Protocols
Reviews (2)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A549 cells, A431 cells, C6 cells, HeLa cells, HepG2 cells, MCF-7 cells, MDA-MB-453s cells, multi-cells, U-937 cells, K-562 cells, NIH/3T3 cells, Calu-1 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon tissue, human kidney tissue, human lung tissue, human oesophagus tissue, human ovary cancer tissue, human ovary tumor tissue, human small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells, A431 cells
Positive FC (Intra) detected in	NIH/3T3 cells, Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.50 ug per 10^6 cells in a 100 ul suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21990-1-AP targets Annexin A1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, bovine, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17273 Product name: Recombinant human ANXA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-346 aa of BC001275 Sequence: MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN Predict reactive species
Full Name	annexin A1
Calculated Molecular Weight	346 aa, 39 kDa
Observed Molecular Weight	37 kDa
GenBank Accession Number	BC001275
Gene Symbol	Annexin A1
Gene ID (NCBI)	301
RRID	AB_11182596
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P04083
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.

Protocols

Product Specific Protocols
FC protocol for Annexin A1 antibody 21990-1-AP	Download protocol
IF protocol for Annexin A1 antibody 21990-1-AP	Download protocol
IHC protocol for Annexin A1 antibody 21990-1-AP	Download protocol
IP protocol for Annexin A1 antibody 21990-1-AP	Download protocol
WB protocol for Annexin A1 antibody 21990-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The Annexin A1 Polyclonal antibody (Cat# 21990-1-AP) has 72 citations across journals including Nature, Cell, Nat Commun, J Clin Invest, Sci Adv, Oncogene, Cell Death Dis, FASEB J, PLoS Pathog, and iScience. Research fields span oncology (glioma, melanoma, breast, lung, gastric, thyroid, and cholangiocarcinoma), immunology and inflammation, extracellular vesicle biology, metabolism (fatty acid, cholesterol, glucose), neuroscience, infectious disease, wound healing, liver and kidney injury, and diabetes. Applications include WB, IF, IHC, IP, and CoIP across human, mouse, rat, pig, bovine, and hamster species.

Species	Application	Title
mouse	WB,IF	Nature Evolutionarily divergent mTOR remodels translatome for tissue regeneration Authors - Olena Zhulyn View Article
human	IF	Cell A pan-cancer single-cell panorama of human natural killer cells Authors - Fei Tang View Article
	IP	Cell Host Microbe Global mapping of Salmonella enterica-host protein-protein interactions during infection. Authors - Philipp Walch View Article
		J Extracell Vesicles USP5-Rich Apoptotic Extracellular Vesicles Regulate Nucleus Pulposus Cells Apoptosis and DNA Damage Repair by Preventing E2F1 Proteasomal Degradation Authors - Pengzhi Shi View Article
mouse	WB	J Extracell Vesicles Magnetic-Assisted Aptamer Selection and Isolation of Migrasomes for Application in Lung Adenocarcinoma Proteomic Analysis. Authors - Yixin Xu View Article
human	IF	Nat Commun Annexin A1 is a polarity cue that directs mitotic spindle orientation during mammalian epithelial morphogenesis Authors - Maria Fankhaenel View Article

Reviews

4.5 out of 5

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2 verified customer reviews

What customers say

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Emily (Verified Customer) (07-23-2025)	The antibody works well in differentiating microvesicles from exosomes when determining the purity of our EV isolations. Applications: Western Blot Primary Antibody Dilution: 1:5000 Cell Tissue Type: LOX, 786-O
Guorong (Verified Customer) (05-23-2022)	excellent with 293T cells Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: 293T Annexin A1 Antibody Western Blot validation (1:2000 dilution) in 293T (Cat no:21990-1-AP)