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Annexin A1 Polyclonal antibody
Annexin A1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 21990-1-AP
|Positive WB detected in||PC-3 cells, multi-cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human oesophagus tissue, human kidney tissue, human small intestine tissue, human ovary tumor tissue, human lung tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A431 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21990-1-AP is Annexin A1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||bovine, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Annexin A1 fusion protein Ag17273|
|Full Name||annexin A1|
|Calculated molecular weight||346 aa, 39 kDa|
|Observed molecular weight||37 kDa|
|GenBank accession number||BC001275|
|Gene ID (NCBI)||301|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.
|Product Specific Protocols|
|WB protocol for Annexin A1 antibody 21990-1-AP||Download protocol|
|IHC protocol for Annexin A1 antibody 21990-1-AP||Download protocol|
|IF protocol for Annexin A1 antibody 21990-1-AP||Download protocol|
|IP protocol for Annexin A1 antibody 21990-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Cell Physiol
TCEA3 promotes differentiation of C2C12 cells via an Annexin A1-mediated transforming growth factor-β signaling pathway.
Biochem Biophys Res Commun
Participation of delta annexin A3 in the ribosomal protein S19 C-terminus-dependent inhibitory mechanism of the neutrophil C5a receptor through delta lactoferrin.
Mol Reprod Dev
Proteomic analysis of seminal extracellular vesicle proteins involved in asthenozoospermia by iTRAQ.
Mol Cell Biochem
WISP1 promotes bovine MDSC differentiation via recruitment of ANXA1 for the regulation of the TGF-β signalling pathway.
ANXA1 affects murine hair follicle growth through EGF signaling pathway.