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  • KD/KO Validated

Annexin A1 Polyclonal antibody

Annexin A1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21990-1-AP

Synonyms

Annexin 1, annexin A1, Annexin I, ANX1, ANXA1, Calpactin 2, Calpactin II, Chromobindin 9, Lipocortin I, LPC1



Tested Applications

Positive WB detected inPC-3 cells, multi-cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman oesophagus tissue, human kidney tissue, human small intestine tissue, human ovary tumor tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21990-1-AP is Annexin A1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity bovine, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin A1 fusion protein Ag17273
Full Name annexin A1
Calculated molecular weight 346 aa, 39 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC001275
Gene symbol ANXA1
Gene ID (NCBI) 301
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.

Protocols

Product Specific Protocols
WB protocol for Annexin A1 antibody 21990-1-APDownload protocol
IHC protocol for Annexin A1 antibody 21990-1-APDownload protocol
IF protocol for Annexin A1 antibody 21990-1-APDownload protocol
IP protocol for Annexin A1 antibody 21990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF,IP

J Cell Physiol

TCEA3 promotes differentiation of C2C12 cells via an Annexin A1-mediated transforming growth factor-β signaling pathway.

Authors - Yao Ge
  • KD Validated
humanWB,IHC

Biochem Biophys Res Commun

PMID: 30292410

Authors - Chuiming Jia
  • KD Validated
humanWB

Cell Death Dis

Exosomal annexin A6 induces gemcitabine resistance by inhibiting ubiquitination and degradation of EGFR in triple-negative breast cancer.

Authors - Ting Li
humanWB

Pathol Int

Participation of delta annexin A3 in the ribosomal protein S19 C-terminus-dependent inhibitory mechanism of the neutrophil C5a receptor through delta lactoferrin.

Authors - Koji Yamanegi
mouseWB

Mol Reprod Dev

Proteomic analysis of seminal extracellular vesicle proteins involved in asthenozoospermia by iTRAQ.

Authors - Yu Lin
bovineWB,IF,IP

Mol Cell Biochem

WISP1 promotes bovine MDSC differentiation via recruitment of ANXA1 for the regulation of the TGF-β signalling pathway.

Authors - Chunyu Zhang
  • KD Validated