Annexin A1 Polyclonal antibody, PBS Only

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• Annexin A1 Polyclonal antibody, PBS Only (21990-1-PBS)

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  WB analysis using 21990-1-AP (same clone as 21990-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 21990-1-AP (same clone as 21990-1-PBS)

  WB result of Annexin A1 antibody (21990-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A1 transfected HeLa cells. This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21990-1-AP (same clone as 21990-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21990-1-AP (same clone as 21990-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  WB analysis of multi-cells using 21990-1-AP (same clone as 21990-1-PBS)

  WB result of 21990-1-AP (Annexin A1 antibody). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

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  WB analysis of MDA-MB-453s using 21990-1-AP (same clone as 21990-1-PBS)

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 21990-1-AP (same clone as 21990-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 21990-1-AP (same clone as 21990-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 21990-1-AP (same clone as 21990-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 21990-1-AP (same clone as 21990-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 21990-1-AP (same clone as 21990-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 21990-1-AP (same clone as 21990-1-PBS)

  IP result of anti-Annexin A1 (IP:21990-1-AP, 4ug; Detection:21990-1-AP 1:20000) with HeLa cells lysate 1120 ug. This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 21990-1-AP (same clone as 21990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21990-1-AP (Annexin A1 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 21990-1-AP (same clone as 21990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21990-1-AP (Annexin A1 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus using 21990-1-AP (same clone as 21990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus using 21990-1-AP (same clone as 21990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 21990-1-AP (same clone as 21990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21990-1-AP (Annexin A1 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 21990-1-AP (same clone as 21990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 21990-1-AP (same clone as 21990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 21990-1-AP (same clone as 21990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 21990-1-AP (same clone as 21990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 21990-1-AP (same clone as 21990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 21990-1-AP (same clone as 21990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 21990-1-AP (same clone as 21990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 21990-1-AP (same clone as 21990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus using 21990-1-AP (same clone as 21990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus using 21990-1-AP (same clone as 21990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 21990-1-AP (same clone as 21990-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Annexin A1 antibody (21990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 21990-1-AP (same clone as 21990-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Annexin A1 antibody (21990-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  FC experiment of NIH/3T3 using 21990-1-AP (same clone as 21990-1-PBS)

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Human Annexin A1 (21990-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 21990-1-AP (same clone as 21990-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Annexin A1 Polyclonal antibody (21990-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 21990-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Annexin A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 21990-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

ANXA1, Annexin 1, Annexin Ac2-26, Annexin I, Annexin-1

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Product Information

21990-1-PBS targets Annexin A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17273

  Product name: Recombinant human ANXA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-346 aa of BC001275

  Sequence: MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN

• Full Name: annexin A1
• Calculated Molecular Weight: 346 aa, 39 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC001275
• Gene Symbol: Annexin A1
• Gene ID (NCBI): 301
• RRID: AB_11182596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04083
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.