- Featured Product
- KD/KO Validated
Annexin A2 Polyclonal antibody
Annexin A2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (3)
WB, RIP, IP, IHC, IF, FC, ELISA
Cat no : 11256-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, HEK-293 cells, mouse placenta tissue, HepG2 cells, NIH/3T3 cells, mouse kidney tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human liver tissue, human hepatocirrhosis tissue, human lung cancer tissue, human oesophagus cancer tissue, human prostate cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||T-47D cells, MCF-7 cells, HeLa cells|
|Positive FC detected in||K-562 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 7 publications below|
|WB||See 41 publications below|
|IHC||See 14 publications below|
|IF||See 11 publications below|
|IP||See 6 publications below|
|RIP||See 2 publications below|
11256-1-AP targets Annexin A2 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, chicken, monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Annexin A2 fusion protein Ag1777|
|Full Name||annexin A2|
|Calculated molecular weight||339 aa, 39 kDa|
|Observed molecular weight||35-40 kDa|
|GenBank accession number||BC016774|
|Gene ID (NCBI)||302|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.
|Product Specific Protocols|
|WB protocol for Annexin A2 antibody 11256-1-AP||Download protocol|
|IHC protocol for Annexin A2 antibody 11256-1-AP||Download protocol|
|IF protocol for Annexin A2 antibody 11256-1-AP||Download protocol|
|IP protocol for Annexin A2 antibody 11256-1-AP||Download protocol|
|Click here to view our Standard Protocols|
NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.
p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.
Nat Chem Biol
YAP-dependent proliferation by a small molecule targeting annexin A2.
Nucleic Acids Res
Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.
Cell Mol Immunol
CD147 deficiency in T cells prevents thymic involution by inhibiting the EMT process in TECs in the presence of TGFβ.