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  • KD/KO Validated

Annexin A2 Polyclonal antibody

Annexin A2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, RIP, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11256-1-AP

Synonyms

Annexin 2, annexin A2, Annexin II, ANX2, ANX2L4, ANXA2, CAL1H, Calpactin 1 heavy chain, Calpactin I heavy chain, Chromobindin 8, LIP2, Lipocortin II, LPC2, LPC2D, P36, PAP IV, Protein I



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, NIH/3T3 cells, mouse placenta tissue, rat uterus tissue, nouse kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue, human breast cancer tissue, human prostate cancer tissue, human oesophagus cancer tissue, human colon cancer tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inT-47D cells, HeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

11256-1-AP targets Annexin A2 in WB, RIP, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, human, monkey, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin A2 fusion protein Ag1777
Full Name annexin A2
Calculated molecular weight 339 aa, 39 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC016774
Gene symbol ANXA2
Gene ID (NCBI) 302
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

Protocols

Product Specific Protocols
WB protocol for Annexin A2 antibody 11256-1-APDownload protocol
IHC protocol for Annexin A2 antibody 11256-1-APDownload protocol
IF protocol for Annexin A2 antibody 11256-1-APDownload protocol
IP protocol for Annexin A2 antibody 11256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Biochem Biophys Res Commun

Annexin A2 is involved in activation of extracellular signal-regulated kinase upon endothelin-1 stimulation.

Authors - Yuichi Mazaki
humanWB,IHC

Int J Mol Med

Expression of annexin II and stromal tenascin C promotes epithelial to mesenchymal transition and correlates with distant metastasis in pancreatic cancer.

Authors - Naoko Yoneura
  • KD Validated
humanIF,IHC

EBioMedicine

Compounds targeting YadC of uropathogenic Escherichia coli and its host receptor annexin A2 decrease bacterial colonization in bladder.

Authors - Xiao Li
humanWB,IP

Biomolecules

(20S)G-Rh2 Inhibits NF-κB Regulated Epithelial-Mesenchymal Transition by Targeting Annexin A2.

Authors - Yu-Shi Wang
humanIHC

ACS Biomater Sci Eng

Matrisome Provides a Supportive Microenvironment for Skin Functions of Diverse Species.

Authors - Binghui Liu
humanWB,IF,IP

Cell Death Dis

TRIM21-regulated Annexin A2 plasma membrane trafficking facilitates osteosarcoma cell differentiation through the TFEB-mediated autophagy.

Authors - Huan-Tian Zhang