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Annexin A2 Polyclonal antibody
Annexin A2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, RIP, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 11256-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, mouse placenta tissue, HepG2 cells, NIH/3T3 cells, mouse kidney tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human liver tissue, human hepatocirrhosis tissue, human lung cancer tissue, human oesophagus cancer tissue, human prostate cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | T-47D cells, MCF-7 cells, HeLa cells |
Positive FC detected in | K-562 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 7 publications below |
WB | See 41 publications below |
IHC | See 14 publications below |
IF | See 11 publications below |
IP | See 6 publications below |
RIP | See 2 publications below |
Product Information
11256-1-AP targets Annexin A2 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, monkey, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Annexin A2 fusion protein Ag1777 |
Full Name | annexin A2 |
Calculated molecular weight | 339 aa, 39 kDa |
Observed molecular weight | 35-40 kDa |
GenBank accession number | BC016774 |
Gene symbol | ANXA2 |
Gene ID (NCBI) | 302 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.
Protocols
Product Specific Protocols | |
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WB protocol for Annexin A2 antibody 11256-1-AP | Download protocol |
IHC protocol for Annexin A2 antibody 11256-1-AP | Download protocol |
IF protocol for Annexin A2 antibody 11256-1-AP | Download protocol |
IP protocol for Annexin A2 antibody 11256-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Immunol NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death. | ||
Nat Chem Biol YAP-dependent proliferation by a small molecule targeting annexin A2.
| ||
Nucleic Acids Res Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing. | ||
Cell Mol Immunol CD147 deficiency in T cells prevents thymic involution by inhibiting the EMT process in TECs in the presence of TGFβ. |