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- KD/KO Validated
Annexin A2 Polyclonal antibody
Annexin A2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 11256-1-AP
|Positive WB detected in||HeLa cells, HEK-293 cells, HepG2 cells, NIH/3T3 cells, mouse placenta tissue, rat uterus tissue, nouse kidney tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human liver tissue, human breast cancer tissue, human prostate cancer tissue, human oesophagus cancer tissue, human colon cancer tissue, human hepatocirrhosis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||T-47D cells, HeLa cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
11256-1-AP targets Annexin A2 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||chicken, human, monkey, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Annexin A2 fusion protein Ag1777|
|Full Name||annexin A2|
|Calculated molecular weight||339 aa, 39 kDa|
|Observed molecular weight||35-40 kDa|
|GenBank accession number||BC016774|
|Gene ID (NCBI)||302|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.
|Product Specific Protocols|
|WB protocol for Annexin A2 antibody 11256-1-AP||Download protocol|
|IHC protocol for Annexin A2 antibody 11256-1-AP||Download protocol|
|IF protocol for Annexin A2 antibody 11256-1-AP||Download protocol|
|IP protocol for Annexin A2 antibody 11256-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biochem Biophys Res Commun
Annexin A2 is involved in activation of extracellular signal-regulated kinase upon endothelin-1 stimulation.
Int J Mol Med
Expression of annexin II and stromal tenascin C promotes epithelial to mesenchymal transition and correlates with distant metastasis in pancreatic cancer.
Compounds targeting YadC of uropathogenic Escherichia coli and its host receptor annexin A2 decrease bacterial colonization in bladder.
(20S)G-Rh2 Inhibits NF-κB Regulated Epithelial-Mesenchymal Transition by Targeting Annexin A2.
ACS Biomater Sci Eng
Matrisome Provides a Supportive Microenvironment for Skin Functions of Diverse Species.
Cell Death Dis
TRIM21-regulated Annexin A2 plasma membrane trafficking facilitates osteosarcoma cell differentiation through the TFEB-mediated autophagy.