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  • KD/KO Validated

Annexin A2 Polyclonal antibody

Annexin A2 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (4)





Cat no : 11256-1-AP


Annexin 2, annexin A2, Annexin II, ANX2, ANX2L4, ANXA2, CAL1H, Calpactin 1 heavy chain, Calpactin I heavy chain, Chromobindin 8, LIP2, Lipocortin II, LPC2, LPC2D, P36, PAP IV, Protein I

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, mouse placenta tissue, HepG2 cells, NIH/3T3 cells, mouse kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue, human hepatocirrhosis tissue, human lung cancer tissue, human oesophagus cancer tissue, human prostate cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inT-47D cells, MCF-7 cells, HeLa cells
Positive FC detected inK-562 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11256-1-AP targets Annexin A2 in WB, IP, IF, FC, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, monkey, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin A2 fusion protein Ag1777
Full Name annexin A2
Calculated Molecular Weight 339 aa, 39 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC016774
Gene Symbol ANXA2
Gene ID (NCBI) 302
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.


Product Specific Protocols
WB protocol for Annexin A2 antibody 11256-1-APDownload protocol
IHC protocol for Annexin A2 antibody 11256-1-APDownload protocol
IF protocol for Annexin A2 antibody 11256-1-APDownload protocol
IP protocol for Annexin A2 antibody 11256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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  • KD Validated

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Ioannis (Verified Customer) (09-13-2023)

Works perfectly for Zebrafish (IHC).

  • Applications: Immunohistochemistry