Annexin A2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Annexin A2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, RIP, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

55

Cat no : 11256-1-AP

Synonyms

Annexin 2, annexin A2, Annexin II, ANX2, ANX2L4, ANXA2, CAL1H, Calpactin 1 heavy chain, Calpactin I heavy chain, Chromobindin 8, LIP2, Lipocortin II, LPC2, LPC2D, P36, PAP IV, Protein I

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Validation Data Gallery

• 

  WB analysis using 11256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11256-1-AP

  WB result of Annexin A2 (11256-1-AP, 1:1000) with si-Control and si-Annexin A2 transfected HEK293 cells.

• 

  WB analysis of mouse placenta using 11256-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat uterus using 11256-1-AP

  rat uterus tissue were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11256-1-AP

  IP result of anti-Annexin A2 (IP:11256-1-AP, 4ug; Detection:11256-1-AP 1:3000) with HeLa cells lysate 2640 ug.

• 

  IHC staining of human liver using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11256-1-AP (Annexin A2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11256-1-AP (Annexin A2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of T-47D using 11256-1-AP

  Immunofluorescent analysis of (4% PFA) fixed T-47D cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 11256-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 11256-1-AP (Annexin A2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 11256-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11256-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11256-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of K-562 using 11256-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Annexin A2 (11256-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, mouse placenta tissue, HepG2 cells, NIH/3T3 cells, mouse kidney tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver tissue, human hepatocirrhosis tissue, human lung cancer tissue, human oesophagus cancer tissue, human prostate cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: T-47D cells, MCF-7 cells, HeLa cells
• Positive FC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 7 publications below
• WB: See 41 publications below
• IHC: See 14 publications below
• IF: See 11 publications below
• IP: See 6 publications below
• RIP: See 2 publications below

Product Information

11256-1-AP targets Annexin A2 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, monkey, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Annexin A2 fusion protein Ag1777
• Full Name: annexin A2
• Calculated molecular weight: 339 aa, 39 kDa
• Observed molecular weight: 35-40 kDa
• GenBank accession number: BC016774
• Gene symbol: ANXA2
• Gene ID (NCBI): 302
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

Publications

Species	Application	Title
human		Nat Immunol; NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.; Authors - Di Huang
human	WB	Sci Adv; p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.; Authors - Xiaochao Tan
human	WB	Nat Chem Biol; YAP-dependent proliferation by a small molecule targeting annexin A2.; Authors - Sophia Z Shalhout ; KD Validated
human	WB	Nat Commun; PMID: 31792210; Authors - Sunwang Xu
mouse	WB	Nucleic Acids Res; Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.; Authors - Ken Asada
mouse	IF	Cell Mol Immunol; CD147 deficiency in T cells prevents thymic involution by inhibiting the EMT process in TECs in the presence of TGFβ.; Authors - Ruo Chen

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