Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, mouse placenta tissue, THP-1 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse placenta tissue, human bowen disease tissue, human breast cancer tissue, human hepatocirrhosis tissue, human liver tissue, human lung cancer tissue, human oesophagus cancer tissue, human prostate cancer tissue, mouse kidney tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | K-562 cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11256-1-AP targets Annexin A2 in WB, IHC, IF, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey, chicken, zebrafish, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1777 Product name: Recombinant human Annexin A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-339 aa of BC016774 Sequence: MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD Predict reactive species |
| Full Name | annexin A2 |
| Calculated Molecular Weight | 339 aa, 39 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC016774 |
| Gene Symbol | Annexin A2 |
| Gene ID (NCBI) | 302 |
| ENSEMBL Gene ID | ENSG00000182718 |
| RRID | AB_2057311 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07355 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Annexin A2 antibody 11256-1-AP | Download protocol |
| IF protocol for Annexin A2 antibody 11256-1-AP | Download protocol |
| IHC protocol for Annexin A2 antibody 11256-1-AP | Download protocol |
| IP protocol for Annexin A2 antibody 11256-1-AP | Download protocol |
| WB protocol for Annexin A2 antibody 11256-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Annexin A2 Polyclonal antibody (Cat# 11256-1-AP) has 112 citations across journals including Nature Metabolism, Nature Communications, Nature Immunology, Cell Molecular Immunology, Nature Chemical Biology, Advanced Science, Cell Death & Differentiation, Oncogene, Science Advances, and Molecular Cancer. Research fields span cancer biology (hepatocellular, pancreatic, ovarian, breast, prostate, and lung cancers), immunology, neurology, virology, autophagy, ferroptosis, exosome biology, diabetes, cardiovascular disease, and veterinary medicine, with applications in WB, IHC, IF, IP, and ELISA.
| Species | Application | Title |
|---|---|---|
Mol Cancer Human epididymis protein 4 in association with Annexin II promotes invasion and metastasis of ovarian cancer cells.
| ||
Nat Metab HK1 from hepatic stellate cell-derived extracellular vesicles promotes progression of hepatocellular carcinoma | ||
Nat Immunol NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death. | ||
Cell Mol Immunol CD147 deficiency in T cells prevents thymic involution by inhibiting the EMT process in TECs in the presence of TGFβ. | ||
Nat Commun Erythroid-intrinsic activation of TLR8 impairs erythropoiesis in inherited anemia |
Reviews
What customers say
Customers report positive results with this antibody across multiple applications. One user achieved clear Western Blot signals on RAW 264.7 mouse macrophage cell lysate using a 1:1000 dilution with a 1-hour room-temperature incubation, rating it 4 out of 5. Another user confirmed excellent performance for Immunohistochemistry on Zebrafish tissue, giving it a full 5 out of 5 and noting it "works perfectly." Overall, the antibody is well-received for both WB and IHC, with demonstrated reactivity in mouse and zebrafish samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lianne (Verified Customer) (10-22-2025) | 50ug of whole RAW 254.7 cell lysate run on 12% SDS-PAGE gel and transferred onto PVDF. Blot incubated for 1 hour at room temperature with 1:1000 anti-AnnexinA2.
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Ioannis (Verified Customer) (09-13-2023) | Works perfectly for Zebrafish (IHC).
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