APH1A Recombinant monoclonal antibody

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• APH1A Recombinant monoclonal antibody (87181-1-RR)

• 

  WB analysis using 87181-1-RR

  U-937 cells were subjected to SDS PAGE followed by western blot with 87181-1-RR (APH1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of U-937 using 87181-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U-937 cells using APH1A antibody (87181-1-RR, Clone: 251353F8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

APH1A Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87181-1-RR

Clone No.251353F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

APH 1A, Aph 1alpha, APH-1a, Aph-1alpha, CGI 78

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Tested Applications

• Positive WB detected in: U-937 cells
• Positive IF/ICC detected in: U-937 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87181-1-RR targets APH1A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: anterior pharynx defective 1 homolog A (C. elegans)
• Calculated Molecular Weight: 265 aa, 29 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC015568
• Gene Symbol: APH1A
• Gene ID (NCBI): 51107
• RRID: AB_3745395
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96BI3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APH1A is a component of the gamma secretase complex that cleaves integral membrane proteins such as Notch receptors and beta-amyloid precursor protein. The gamma secretase complex contains this gene product, or the paralogous anterior pharynx defective 1 homolog B (APH1B), along with the presenilin, nicastrin, and presenilin enhancer-2 proteins. The precise function of this seven-transmembrane-domain protein is unknown though it is suspected of facilitating the association of nicastrin and presenilin in the gamma secretase complex as well as interacting with substrates of the gamma secretase complex prior to their proteolytic processing. Polymorphisms in a promoter region of this gene have been associated with an increased risk for developing sporadic Alzheimer's disease.