CoraLite® Plus 647-conjugated APOE Monoclonal antibody

APOE Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1B2C9

Cat no : CL647-66830

Synonyms

AD2, Apo E, APOE, apolipoprotein E, LPG

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Validation Data Gallery

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  FC experiment of HepG2 using CL647-66830

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human APOE (CL647-66830, Clone:1B2C9) (red), or 0.2 ug APC-65124 ; APC Mouse IgG1 Isotype Control (APC-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: HepG2 cells

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Product Information

CL647-66830 targets APOE in FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: APOE fusion protein Ag28186
• Full Name: apolipoprotein E
• Calculated molecular weight: 36 kDa
• Observed molecular weight: 34-36 kDa
• GenBank accession number: BC003557
• Gene symbol: APOE
• Gene ID (NCBI): 348
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serve as a ligand for the LDL (APO B/E) receptor and for the specific APOE receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.