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APPL1 Monoclonal antibody

APPL1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B7B11

Cat no : 68195-1-Ig

Synonyms

APPL, APPL1, Dip13 alpha, DIP13A, DIP13alpha, KIAA1428



Tested Applications

Positive WB detected inLNCaP cells, Jurkat cells, rabbit heart tissue, HepG2 cells, pig heart tissue, HEK-293 cells, HeLa cells, rat heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68195-1-Ig targets APPL1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen APPL1 fusion protein Ag3334
Full Name adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Calculated Molecular Weight 709 aa, 80 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC028599
Gene Symbol APPL1
Gene ID (NCBI) 26060
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of INS signaling, plays a key role in the regulation of INS secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the INS-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].

Protocols

Product Specific Protocols
WB protocol for APPL1 antibody 68195-1-IgDownload protocol
IHC protocol for APPL1 antibody 68195-1-IgDownload protocol
IF protocol for APPL1 antibody 68195-1-IgDownload protocol
FC protocol for APPL1 antibody 68195-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols